Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

aSince 1000 ml of 0.2 M acetic acid contain¼0.2 moles, so 46 ml of 0.2 M acetic acid will contain¼0.246/1000¼ 9200 μmoles Calcu ...

S. no Volume of 0.2 M Tris taken Amount of 0.2 M Tris in used volume (μmole) Volume of 0.2 M HCl taken Amount of 0.2 M HCl in us ...

Examination of Urine for Normal Constituents^7 Urine is the excretory product of the body produced by the process offiltration, ...

7.2 Physical Examination of Urine 7.2.1 Color and Odor Normal urine is colorless to straw colored due to presence of urochrome. ...

7.2.5 pH Normal range of urinary pH is 4.5–7.5. Various factors like heavy meals, heavy exercise, metabolic acidosis, or chronic ...

Phosphates To 3 ml of urine, add 1 ml of conc. HNO 3 and 5 ml of ammonium molybdate and warm gently. A yellow precipitate of pho ...

To Perform Qualitative Tests for Urinary Proteins^8 8.1 Theory Proteins are organic compounds containing nitrogen in addition to ...

8.2 Tests for Urinary Proteins 8.2.1 Dipstick Test This is the most common test for proteinuria. Dipsticks are plastic strips im ...

8.2.3 Sulphosalicylic Acid Test Sulphosalicylic acid is an anionic protein precipitant; it reacts with protein cations and cause ...

is increased further, then precipitates get dissolved, and when cooled back to 40 – 60 C, the proteins precipitate again. Preca ...

amount of albumin is observed in urine in insulin-dependent diabetics, and this finding appears to correlate with very early dia ...

To Determine the Quantity of Proteins in Urine Sample Using Biuret Reaction^9 9.1 Theory Healthy persons excrete urine that is l ...

form a coordination complex with four imino groups present in the peptide bonds and show an absorption maximum at 540 nm. The me ...

Distilled water (ml) 1 0.8 0.6 0.4 0.2 – 0.8 20% TCA (ml) – 111111 Mix and incubate all the tubes at 37C for 30 min. Centrifuge ...

To Estimate the Amount of Total Protein and Albumin in Serum and to Find A/G Ratio 10 10.1 Theory Proteins are present in all bo ...

compound biuret, and therefore the reaction is called the biuret reaction. Amino acids and dipeptides do not give this reaction, ...

4.Working protein standard:Dilute stock standard with normal saline to get concentration range of 2, 4, 6, 8, and 10 g/dl. 10.5 ...

10.7 Estimation of Albumin in Serum Method Bromocresol Green (BCG) Binding 10.8 Principle....................................... ...

In tube T, add 0.02 ml of serum sample and in blank add 0.02 ml of distilled water. Then add 4 ml of BCG solution in all the tu ...

reducing plasma-albumin formation. Albumin concentration decreases due to loss in urine in nephrotic syndrome, chronic glomerula ...