Nature - USA (2020-09-24)

Article Methods Histone expression and purification Xenopus laevis histones were individually overexpressed in BL21(DE3) pLysS b ...

bridging assay. Incorporation of biotin prevented continuous DNA end joining and formation of ladders of PARP2–DNA complex speci ...

Article windowed and 2D class averages were generated with the Relion software package^40. Inconsistent class averages were remo ...

Extended Data Fig. 1 | Assembly and cryo-EM of PARP2–HPF1 bound to mononucleosomes. a, b, SDS–PAGE (a) and native gel (b) showin ...

Article Extended Data Fig. 2 | See next page for caption. ...

Extended Data Fig. 2 | Classif ication of the PARP2–HPF1–nucleosome complex. a, Initial map generated from the entire data set, ...

Article Extended Data Fig. 3 | See next page for caption. ...

Extended Data Fig. 3 | Focused classif ication, ref inement and model building: focus on the PARP2–HPF1 complex. a, The overall ...

Article Extended Data Fig. 4 | PARP2 interaction with nucleosomes. a, The PARP2– HPF1 subset comprising 140,000 particles (left) ...

Extended Data Fig. 5 | Interaction of HPF1 with the nucleosome stabilizes the PARP2–HPF1–nucleosome complex. a, Native gel showi ...

Article Extended Data Fig. 6 | See next page for caption. ...

Extended Data Fig. 6 | Bridging of two nucleosomes is required for PARP2 activation. a, PARP2 can bind double-stranded DNA with ...

Article Extended Data Fig. 7 | Bridging of two nucleosomes induces conformational changes in PARP2. a, Alignment of WGR domains ...

Extended Data Fig. 8 | See next page for caption. ...

Article Extended Data Fig. 8 | Model for PARP2–HPF1 in open state 1. a, Model of activated PARP2–HPF1 bound to nucleosome (Fig.  ...

Extended Data Fig. 9 | See next page for caption. ...

Article Extended Data Fig. 9 | PARP2–HPF1 in open state 2. a, Model of the disordered H3 N-terminal tail bound to the active sit ...

Extended Data Fig. 10 | Mutations that cause resistance to PARP inhibitors. a, Linker histone H1 is accessible for PARylation wh ...

Article Extended Data Table 1 | Cryo-EM data collection, refinement and validation statistics ...

1 nature research | reporting summary April 2020 Corresponding author(s): Mario Halic Last updated by author(s):Jun 9, 2020 Repo ...