Nature - USA (2020-09-24)

2 nature research | reporting summary April 2020 Field-specific reporting Please select the one below that is the best fit for y ...

614 | Nature | Vol 585 | 24 September 2020 Article Biosynthesis of medicinal tropane alkaloids in yeast Prashanth Srinivasan^1 & ...

Nature | Vol 585 | 24 September 2020 | 615 precursors in yeast (Fig. 1a). Modules I/II and III enable de novo biosyn- thesis of ...

616 | Nature | Vol 585 | 24 September 2020 Article mechanisms encoded by methylthioadenosine phosphorylase (Meu1) and ornithine ...

Nature | Vol 585 | 24 September 2020 | 617 from fed littorine (m/z 290 [M + H]+) via a three-step biosynthetic pathway (Fig. 1a) ...

618 | Nature | Vol 585 | 24 September 2020 Article (eGFP, tagBFP, mVenus) and Discosoma (mCherry, DsRed.T3); small ubiquitin-rel ...

Nature | Vol 585 | 24 September 2020 | 619 Our demonstration of total biosynthesis of hyoscyamine and scopola- mine via engineer ...

Article Methods Data reporting No statistical methods were used to predetermine sample size. The experiments were not randomized ...

Growth conditions for metabolite assays Small-scale metabolite production assays were performed in YNB-SC or YNB-DO media supple ...

Article functional annotations for each of 43,861 unique transcripts identi- fied from the A. belladonna transcriptome were obta ...

All infiltrations were performed in triplicate, in which one biological replicate comprised three infiltrated leaves from a sing ...

Article Källberg, M. et al. Template-based protein structure modeling using the RaptorX web server. Nat. Protoc. 7 , 1511–1522 ...

Extended Data Fig. 1 | Design of genomic integrations for pathway construction in yeast. Block arrows represent gene expression ...

Article Extended Data Fig. 2 | Substrate specif icity and structure-guided active site engineering of UGT84A27 in engineered yea ...

Extended Data Fig. 3 | See next page for caption. ...

Article Extended Data Fig. 3 | Coexpression analysis, active site mutagenesis, and orthologue identification for AbHDH. a, Heat ...

Extended Data Fig. 4 | Screening H6H orthologues from TA-producing Solanaceae in yeast. H6H orthologues from Anisodus acutangulu ...

Article Extended Data Fig. 5 | Phylogenetic analysis of HDH. a, Sequence alignment of AbHDH, DiHDH and DsHDH (green) with select ...

Extended Data Fig. 6 | See next page for caption. ...

Article Extended Data Fig. 6 | Analysis of AbLS localization, N-glycosylation, and proteolytic processing patterns in yeast and ...