BIOINORGANIC CHEMISTRY A Short Course Second Edition

40 BIOCHEMISTRY FUNDAMENTALS Initial N - terminal and C - terminal sequence determination may be made using end - group analysis ...

PROTEINS 41 of proteins having separate and distinct functions but that are grouped together in a cellular matrix. Proteomics, a ...

42 BIOCHEMISTRY FUNDAMENTALS to known proteins in mixtures, but these are unlikely to provide unequivocal protein identifi catio ...

PROTEINS 43 proteins from which they were derived. The authors stated that proteolytic O^18 labeling enabled a shotgun approach ...

44 BIOCHEMISTRY FUNDAMENTALS with the substrate (substance acted upon) and bind it. Some enzymes require nonprotein molecules or ...

PROTEINS 45 protein ferritin stores iron in the human body. Immunoglobins are proteins that react with and neutralize foreign co ...

46 BIOCHEMISTRY FUNDAMENTALS 111 V K VV = m ()+ max []S max (2.3) Plotting 1/ V vs. 1/[ S ], one obtains a straight line having ...

NUCLEIC ACIDS 47 affi nity for substrate and decrease Vmax while not changing Km. The lines indi- cating competitive and noncomp ...

48 BIOCHEMISTRY FUNDAMENTALS TABLE 2.2 Nitrogenous Bases, Nucleosides, Nucleotides and Sugars Found in DNA and RNA Base a Nucleo ...

NUCLEIC ACIDS 49 Base a Nucleoside Nucleotide Example Sugar NH HN O O 2 4 1 5 Thymine, T 5-methyl uracil Deoxythymidine (DNA) De ...

50 BIOCHEMISTRY FUNDAMENTALS This is described as an antiparallel arrangement. This arrangement allows the two chains to fi t to ...

NUCLEIC ACIDS 51 averaged) structures obtained from X - ray crystallography or NMR. When changing the pucker phase angle from 0 ...

52 BIOCHEMISTRY FUNDAMENTALS Figure 2.16 Orientations found in DNA helices. [Adapted with permission from Figure 2.11 of referen ...

NUCLEIC ACIDS 53 A, B, and Z DNA structure are found in Table 2.3 as adapted from Table 1.10 of reference 15 and the Jena image ...

54 BIOCHEMISTRY FUNDAMENTALS RNA (mRNA) codons needed to synthesize a complete protein. Genes may be collected together in a chr ...

NUCLEIC ACIDS 55 ing free deoxynucleotide triphosphates (dNTPs) to hydrogen bond with their complementary nucleotide on the sing ...

56 BIOCHEMISTRY FUNDAMENTALS and CAC code for histidine (his); GGT/U, GGC, GGA, and GGG code for glycine (gly); T/UAA, T/UAG, an ...

NUCLEIC ACIDS 57 resulting in an incorrect series of amino acids following the frame shift. An example is shown in Figure 2.18. ...

58 BIOCHEMISTRY FUNDAMENTALS such as cytochrome c 551 — electron transfer (et).^18 The authors created two mutant azurins, one i ...

NUCLEIC ACIDS 59 isolated from bacteria, that recognize and cut specifi c sequences — restriction sites — in DNA. One restrictio ...