BIOINORGANIC CHEMISTRY A Short Course Second Edition

120 INSTRUMENTAL METHODS upon enzyme activation; (2) a catalytic domain responsible for the enzyme ’ s hydrolase activity — the ...

NUCLEAR MAGNETIC RESONANCE 121 Figure 3.18 Secondary structure of matrix metalloproteinase 12 (MMP12): (A) PDB: 1YCM (structure ...

122 INSTRUMENTAL METHODS The reference 28 authors concluded that for the NMR solution study of NNGH inhibited MMP12 (MMP12 - NNG ...

ELECTRON PARAMAGNETIC RESONANCE 123 μβ=−g S (3.34) E=⋅μB (3.35) (^) ΔEh=ν (3.36) Replacing μ by its equivalent operator from equ ...

124 INSTRUMENTAL METHODS One can calculate the ratio of populations of spin - up to spin - down electron orientations at room te ...

ELECTRON PARAMAGNETIC RESONANCE 125 the focus here will be on EPR behavior of transition metal centers that occur in biological ...

126 INSTRUMENTAL METHODS For the simple case of one unpaired electron ( S = 1/2), the associated mag- netic moment is not a simp ...

ELECTRON PARAMAGNETIC RESONANCE 127 at low fi eld (from gx and gy , g⊥ or g perpendicular). The spectrum is also said to be axia ...

128 INSTRUMENTAL METHODS magnetic moment. The interactions may be of two types: (1) the hyperfi ne interaction — the unpaired el ...

ELECTRON PARAMAGNETIC RESONANCE 129 distinguish between hyperfi ne and superhyperfi ne coupling in a bioinorganic system where b ...

130 INSTRUMENTAL METHODS what the authors called the purple CyoA protein.^31 This last protein exhibited different characteristi ...

ELECTRON PARAMAGNETIC RESONANCE 131 coli (labeled PdII in Figure 3.23 ). Figure 3.23 shows the experimental EPR spectra of N 2 O ...

132 INSTRUMENTAL METHODS attached to the C ε atom of histidine), provided spin density and structural information — how the hist ...

velocity at which maximum absorption ofγ rays occurs is determined. For a (^57) Fe source emitting a 14.4 - keV γ ray, the energ ...

134 INSTRUMENTAL METHODS Asymmetry in the ligand environment, either geometric or in charge distri- bution (or both), affect the ...

Table 3.2 lists values for ΔEQ and δ for some important oxidation and spin states found in bioinorganic molecules. Data are take ...

136 INSTRUMENTAL METHODS 7.2.7. Reference 36 provides the Table 3.2 data on iron sulfur clusters found in many bioinorganic spec ...

orientation of the applied fi eld. In other words, the parallel versus perpendicu- lar dependence of the M ö ssbauer spectrum be ...

138 INSTRUMENTAL METHODS interactions (see Sections 3.5.2 and 3.5.3 ) that are too small to be resolved within the natural width ...

OTHER INSTRUMENTAL METHODS 139 Putting together their M ö ssbauer analysis with previous ENDOR data, EXAFS analyses, and X - ray ...