BIOINORGANIC CHEMISTRY A Short Course Second Edition

60 BIOCHEMISTRY FUNDAMENTALS One of the in vitro (in the test tube) processes used to clone DNA is called thepolymerase chain re ...

NUCLEIC ACIDS 61 the double helix. Next, the vial is cooled to 55 ° C so that the primers will bind (anneal) to the DNA strands. ...

62 BIOCHEMISTRY FUNDAMENTALS together to become part of the sequenced genome. The shotgun approach was applied to cloned DNA fra ...

strategies — a whole - genome assembly and a regional chromosome assem- bly — were used, each combining sequence data from Celer ...

64 BIOCHEMISTRY FUNDAMENTALS zinc - fi ngers, that interact with DNA.^24 Zinc ions are required for correct protein folding into ...

TFIIIA zinc - fi nger units and others with very similar aa sequences and DNA binding motifs — Zif268, TTK - 2, YY1 - 3, 1MEY an ...

66 BIOCHEMISTRY FUNDAMENTALS The criteria described have broadened as more zinc - fi nger proteins have become known, so that th ...

2.4.1 Descriptive Examples, One of the fi rst three - dimensional structures of a zinc - fi nger domain, deter- mined by nuclear ...

68 BIOCHEMISTRY FUNDAMENTALS pair subsite. Each of the zinc fi ngers uses arginine, histidine, and aspartic acid amino acid resi ...

GCT - GCG - 3 ′ over the nonpermuted site. The results indicated that observa- tions on the specifi city of zinc fi ngers can be ...

70 BIOCHEMISTRY FUNDAMENTALS Figure 2.23 (A) Amino acid sequence and secondary structure of Zif1, Zif2, and Zif3 zinc fi ngers o ...

DNA interaction follows as adapted from reference 38. The 2DRP X - ray crystallographic structure was solved to 2.8 - Å resoluti ...

72 BIOCHEMISTRY FUNDAMENTALS atoms of two histidines from theα - helical region — his129 and his134 — and the Sγ atoms of two cy ...

in RNA, which will in turn affect contacts between the zinc - fi nger proteins and the nucleic acids. While the binding of Sp1 t ...

74 BIOCHEMISTRY FUNDAMENTALS REFERENCES (a) Nelson , D. L. ; Cox , M. M. Lehninger Principles of Biochemistry , 4th ed. , W. H. ...

Venter , J. C. , et al. Science , 2001 , 291 , 1304 – 1351. Roberts , L. Science , 2001 , 291 , 1182 – 1188. Hanas , J. S. ; Ha ...

76 3 Bioinorganic Chemistry: A Short Course, Second Edition, by Rosette M. Roat-Malone Copyright © 2007 John Wiley & Sons, I ...

red, resonance Raman, circular dichroism, magnetic circular dichroism spec- troscopies, and many others are not discussed here. ...

78 INSTRUMENTAL METHODS Moving an electron from a ground state to an excited state ( Eelectronic ), leading to observations in ...

able for proper diffraction (see Section 3.3 ). X - ray absorption spectroscopy (XAS) can yield limited molecular structural inf ...