BIOINORGANIC CHEMISTRY A Short Course Second Edition

100 INSTRUMENTAL METHODS Substitution and rearrangement lead to equation 3.16 : ν γ π = 2 B 0 (3.16) Values of the magnetogyric ...

NUCLEAR MAGNETIC RESONANCE 101 To produce an observable magnetic effect, the NMR technique resorts to an additional perturbation ...

102 INSTRUMENTAL METHODS and so on, would be gained. However, when a magnetic fi eld is applied during an NMR experiment, electr ...

NUCLEAR MAGNETIC RESONANCE 103 The paramagnetic screening constant becomes disproportionately larger for heavier elements; thus ...

104 INSTRUMENTAL METHODS affects each proton differently, causing larger separation of the resonances. Both effects are useful i ...

NUCLEAR MAGNETIC RESONANCE 105 The valence s electron density at the nucleus. This is affected by the s character of the bondin ...

106 INSTRUMENTAL METHODS The number of nuclei in each letter category is indicated by a subscript. Using the proton as an exampl ...

NUCLEAR MAGNETIC RESONANCE 107 integrate the area under the different peaks in a spectrum containing a number of proton resonanc ...

108 INSTRUMENTAL METHODS nuclear spins are faster and some are slower, the xy magnetization starts to fan out and lose coherence ...

NUCLEAR MAGNETIC RESONANCE 109 tions and their relaxations may be between the same or different type of nucleus, but in either c ...

110 INSTRUMENTAL METHODS than 2.5 Å ), medium (atoms 2.5 – 3.5 Å apart), and weak (atoms 3.5 – 5 Å apart) NOEs. Methods develope ...

NUCLEAR MAGNETIC RESONANCE 111 lengths for each are slightly different. Some distortion of signal intensity results from this fa ...

112 INSTRUMENTAL METHODS Following this, each FID is Fourier - transformed to produce perturbed spectra. These are stacked, and ...

NUCLEAR MAGNETIC RESONANCE 113 timet 1 the magnetization separates into two pairs of vectors due to the chemi- cal shift differe ...

114 INSTRUMENTAL METHODS COSY pulse sequence. The fi rst 90 ° pulse at the^1 H frequency produces trans- versey magnetization. T ...

NUCLEAR MAGNETIC RESONANCE 115 (NOESY) experiment must be used. See Section 3.4.10. The NOESY experi- ment transfers magnetizati ...

116 INSTRUMENTAL METHODS relaxation can also produce an exchange of magnetization through the NOE effect. In either case, nuclei ...

NUCLEAR MAGNETIC RESONANCE 117 have been found to be in excellent agreement with experimentally determined values in solution. M ...

118 INSTRUMENTAL METHODS Horse heart cytochrome c solutions were prepared at concentrations of ∼ 4 – 6 mM in 30% CD 3 CN (deuter ...

NUCLEAR MAGNETIC RESONANCE 119 were converted into distance constraints using the CALIBA module in DYANA (see reference 25b ). T ...