Science - USA (2022-02-11)

Schrieket al.,Science 375 , eabf7470 (2022) 11 February 2022 3 of 12 Fig. 2. MARCH1- deficient mice harbor MZ B cells expressing ...

cDCs (Fig. 5B), even though MARCH1 is functional in these cells as shown by their wild-type expression of CD86 (fig. S4F). To co ...

binding to MHC II has been conserved through evolution. CR2 drives B cell trogocytosis of cDC membranes containing MHC IIÐC3 com ...

MZ B cell trogocytosis occurs constitutively in wild-type mice. Finally, we tested whether trogocytosis enabled MZ B cells to“hi ...

first is cellular and features cDCs and MZ B cells. ThemainroleofcDCsistopresentAgtoTcells andinitiateadaptiveimmunity( 18 ). In ...

enriched by density gradient centrifugation (at 2237g) with 1.077 g/cm^3 Nycodenz (Axis shield) and subsequent negative depletio ...

fluorescence-minus-one (FMO) controls cor- respond to cells that were stained with mAbs for the detection of lineage markers but ...

Enumeration of cells via flow cytometry was performed using a defined number of Sphero blank calibration beads (BD BioSciences). ...

12% Bis-Tris Plus) and transferred to iBlot 2 PVDF membranes with an iBlot 2 system according to manufacturer’s instructions (al ...

Acad. Sci. U.S.A. 93 , 3357–3361 (1996). doi:10.1073/ pnas.93.8.3357; pmid: 8622941 D. Vremec, K. Shortman, The isolation and i ...

RESEARCH ARTICLE SUMMARY ◥ CELL BIOLOGY Mechanism of spindle pole organization and instability in human oocytes Chun So, Katerin ...

RESEARCH ARTICLE ◥ CELL BIOLOGY Mechanism of spindle pole organization and instability in human oocytes Chun So^1 , Katerina Men ...

to the organization of spindle poles in human oocytes. To test directly if NUMA is required for spindle pole organization, we de ...

no longer had NUMA at the spindle poles, and the poles became fully defocused (Fig. 1, G to I). Thus, NUMA is required for pole ...

reexpressed four different NUMA mutants in aMTOC-free mouse oocytes (fig. S6, A to C). NUMA(DN) and NUMA(SpM-4A), which cannot i ...

constitutive and acute depletion of LIS1 pheno- copied dynein-dynactin inhibition and led to spindle pole defocusing in aMTOC-fr ...

Soet al.,Science 375 , eabj3944 (2022) 11 February 2022 6 of 19 Microtubules Dynactin Prophase Nuclear envelope breakdown Onset ...

assembled multipolar spindles and spindles with broad poles, respectively (Fig. 5, F to H; and fig. S14, A and B). By contrast, ...

45% of KIFC1-depleted bovine oocytes under- went tridirectional division (Fig. 6C), which was likely because fewer oocytes resto ...

analysis revealed that wild-type and aMTOC- free mouse oocytes had a comparable fraction of mobile mClover3-KIFC1, but the turno ...