Science - USA (2022-02-11)

11 FEBRUARY 2022 • VOL 375 ISSUE 6581 619 GRAPHIC: KELLIE HOLOSKI/ SCIENCE BASED ON YUN AND GRECO SCIENCE science.org CELL BIOLO ...

620 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 science.org SCIENCE lial cells adopt mesenchymal properties, such as increased migrato ...

11 FEBRUARY 2022 • VOL 375 ISSUE 6581 621 GRAPHIC: KELLIE HOLOSKI/ SCIENCE SCIENCE science.org complex inter-relationship be- tw ...

622 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 PHOTO: WORLD WILDLIFE FUND science.org SCIENCE By William F. Laurance T homas E. Lovej ...

SCIENCE science.org 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 623 ILLUSTRATION: STEFANO MAUGERI/ROYAL SOCIETY OPEN SCIENCE/WIKIMEDIA ...

624 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 science.org SCIENCE By Jennifer Golbeck K elly Weill’s new book, Off the Edge, is a de ...

some beneficial effects of caloric restriction. —LBR Science, abg7292, this issue p. 671; see also abn6576, p. 620 CORONAVIRUS V ...

from the tissue pixels containing single nuclei. Spatial-CUT&Tag adds a new dimension to spatial biology by enabling the map ...

GWAS analysis solely for coffee and tea preferences, which, however, identified specific genetic variants associated with caffei ...

627-B 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 science.org SCIENCE RESEARCH CELL BIOLOGY The making and removal of a leader When ep ...

SCIENCE science.org 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 627-C RESEARCH selecting for dosage compensa- tion and sexually antago ...

RESEARCH ARTICLE SUMMARY ◥ CELL BIOLOGY p53 directs leader cell behavior, migration, and clearance during epithelial repair Kasi ...

RESEARCH ARTICLE ◥ CELL BIOLOGY p53 directs leader cell behavior, migration, and clearance during epithelial repair Kasia Kozyrs ...

p21 promotes the emergence of leader cells downstream of p53 Spontaneous leader cells, BBCs, and MMC- treated cells are all cell ...

(fig. S4, E to J). Induction of leaders by p21 overexpression was also sufficient to up-regulate PI3K and ITGb1(fig.S4,OtoR),whi ...

under the control of p21 promoter sequences that respond to p53 (fig. S6J) ( 30 ). We found that scratching induced p53 activity ...

with high p53 (Fig. 6E), neither did it rescue their loser status in competition experiments with p53 mutant cells (Fig. 6F and ...

Cell culture and plasmids MDCK cells were maintained in Dulbecco’s minimum essential medium (21885, Thermo Fisher Scientific) su ...

used: 5′-CGGCAAGCCTTGCTGCCATG(AGG)- 3 ′and 5′-TGGACAGCGAGCAGCTGCGC(CGG)- 3 ′. sgRNAs were cloned individually into PX461 vectors ...

(PCR) product was introduced into doxycycline- inducible pTRIPZ-RFP-NLS-Puro ( 24 ) with a P2A peptide bridging the two proteins ...