Science - USA (2022-02-11)

Unlike the other two kinesin-14 family mem- bers KIFC2 and KIFC3, KIFC1 distinctively possesses a microtubule-binding domain at ...

KIFC1’s functions in aMTOC-free spindles, we depleted endogenous KIFC1 and reexpressed wild-type KIFC1 and KIFC1(N593K), which c ...

Discussion Our data elucidate a cause of spindle instability in human oocytes: Human oocytes are deficient in the molecular moto ...

Preparation and culture of bovine and porcine oocytes All ovaries were obtained from local slaughter- houses. Bovine and porcine ...

the mRNA for 12 hours before release. For live imaging of the spindle assembly with Trim- Away, bovine oocytes were microinjecte ...

with 4000 U/ml lipase fromCandida rugose (Sigma-Aldrich) in 400 mM NaCl, 50 mM Tris (pH 7.2), 5 mM CaCl 2 , and 0.2% sodium taur ...

transferred into 4ml of protein-free medium at around 6, 15, 11, and 10 hours after NEBD, respectively. Then, 12ml of 1.333× NuP ...

saturation, and original image for brightness. The output images were inverted for better visualization of the results. Reanalys ...

and dynamics in human oocytes, fertilization and early embryos.Mol. Hum. Reprod. 17 , 392–398 (2011). doi:10.1093/molehr/gar009; ...

human preimplantation development.Cell Stem Cell 25 , 697 – 712.e6 (2019). doi:10.1016/j.stem.2019.09.004; pmid: 31588047 Q. Ko ...

GOLDOPENACCESS,DIGITAL,ANDFREETO ALLREADERS AsAAAS’s firstmultidisciplinary, openaccessjournal,ScienceAdvancespublishes research ...

RESEARCH ARTICLES ◥ NEUROSCIENCE Geometry of sequence working memory in macaque prefrontal cortex Yang Xie^1 †, Peiyao Hu^1 †, J ...

particular spatial locations on the ring but also to their ordinal rank in the sequence (first, second, or third). We quantified ...

states for the 18 location-rank combinations by concatenating the regression coefficients of all neurons from correct trials of ...

whether the disentangled representation of sequence memory could be validated at the single-trial level. We tested whether the r ...

scalar at each rank ( 2 ). Rather, gain modu- lation is a collective phenomenon that occurs at the neural population level and i ...

location tuning were anatomically located closer to each other (Fig. 5C). The location code displayed significant clusters at a ...

synfire chains, neural oscillations, or rhythmic fluctuations in neuronal excitability ( 5 , 25 – 27 ). All of these models posi ...

item and order information during a work- ing memory delay ( 33 – 35 ). Our data support such a sustained activity mechanism bec ...

fluid, enabling efficient locomotion. Similarly, in the circulatory system, muscles of the heart dynamically respond to physiolo ...