Science - USA (2022-02-11)

Real-time quantitative reverse-transcription PCR (RT-qPCR) On day 1, 400,000 MDCK cells per well were seeded in a six-well plate ...

The migration speed of scratched epithelial fronts was measured in FIJI by manually drawing freehand lines outlining the wound e ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY CD97 promotes spleen dendritic cell homeostasis through the mechanosensing of red blood ce ...

RESEARCH ARTICLE ◥ IMMUNOLOGY CD97 promotes spleen dendritic cell homeostasis through the mechanosensing of red blood cells Dan ...

Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 2 of 13 A DCIR2 CD8 12.6 74.0 25.2 46.4 19.7 41.8 43.8 28.5 Control Gna ...

contains the GPCR proteolysis site (GPS). The N-terminal fragment (NTF) of CD97 remains noncovalently bound to the seven-transme ...

cDC2s in blood circulation (fig. S2, K and L). Sampling of blood in the splenic artery and vein revealed that cDC2s were most in ...

Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 5 of 13 A 80 60 40 20 0 100 Saline *** 1.5 1.0 0.5 0 2.0 Saline *** B 8 ...

GSEA showed that Mrtf-a–dependent genes ( 29 ) were up-regulated in ArhGEF1- and CD97- deficient cDC2s (Fig. 5E). Many of the Mr ...

cDC2s (Fig. 6A and fig. S7A). In accord with the reduced antigen capture, CD97-deleted cDC2s showed less efficient activation as ...

reactions, potentially resulting in hemolytic dis- eases and renal failure ( 7 , 34 ). Using transfers of foreign antigen conjug ...

further test the contribution of reduced CD97 expression to theIrf4cKOphenotype, we selectively restored CD97 expression in IRF4 ...

cells up-regulate their F-actin, and Mrtf tran- scription factors become active and drive ex- pression of many cytoskeleton-asso ...

BV711-conjugated anti-CD4 (GK1.5), PE-conjugated anti-CD278 (15F9), PE/Cy7-conjugated anti-CD95 (SA367H8), Pacific Blue-conjugat ...

Cell count was analyzed with Imaris automatic spot tracking aided by manual verification, and cell migration was analyzed and ve ...

imaging analysis, J. Muppidi and X. Piao for helpful discussions, and N. Carey for advice on statistical analysis.Funding:D.L. w ...

Where Science Gets Social. AAAS’ MemberCommunityisaone-stopdestinationforscientists and STEM enthusiasts alike. It’s“WhereScienc ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY Marginal zone B cells acquire dendritic cell functions by trogocytosis Patrick Schriek, Al ...

RESEARCH ARTICLE ◥ IMMUNOLOGY Marginal zone B cells acquire dendritic cell functions by trogocytosis Patrick Schriek^1 , Alan C. ...

transfer was observed from B cells to cDCs (fig. S3D). MZ B cells were more trogocytic than their follicular (FO) counterparts ( ...