Cell - 8 September 2016
mutant (KO) versions were used for sorting. 10MUT gp120 KO and 5MUT gp120 KO have mutations T303A, H330A, and N332T; P1981_c5_3 ...
Antibody Cloning and Production Amplified heavy chain and light chain cDNAs were individually cloned into expression vectors con ...
Supplemental Figures 100 80 60 40 20 0 Follicular Frequency (%) Marginal zone Frequency (%) WTGL MutHL^121 HGLL^121 Splenocytes ...
Figure S2. Priming of GLHL121 Knockin Mice, Related toFigure 1 (A) Table shows a list of priming strategies tested in GLHL121 kn ...
Figure S3. Neutralizing Activity in the Serum of Sequentially Immunized GLHL121 and MutHGLL121 Knockin Mice, Related toFigure 3 ...
Figure S4. Immunizations in GLHL121 and MutHGLL121 Mice, Related toFigures 2and 3 (A) Graphs show ELISAs performed with the seru ...
(D) Neutralization activity detected in the serum of GLHL121 and MutHGLL121 mice immunized according to the regimen described in ...
Figure S5. Somatic Mutations in Immunized GLHL121 and MutHGLL121 Mice, Related toFigures 4and 5 (A and B) (A) Diagram of the imm ...
Figure S6. ELISA for Monoclonal Antibodies from Sequentially Immunized Knockin Mice against Engineered and Native-like Proteins, ...
Figure S7. PCR Primers for Antibody Cloning, Related toFigures 4, 5, and 6 (A) Tables show the names of specific primers used fo ...
Article Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies Graphical Abstract Highlights d Design ...
Article Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies Bryan Briney,1,2,3,7Devin Sok,1,2,3,7J ...
germline segments, all known bnAbs share one or more unusual genetic features: very long heavy-chain CDR3 loops; short light- ch ...
native-like N276() trimers. To achieve this goal, we designed ‘‘bridging’’ molecules, which, while still germline-targeting, di ...
(legend on next page) 1462 Cell 166 , 1459–1470, September 8, 2016 ...
antigen-sorted by flow cytometry using the baits listed inFigures 2 C and 2D. When comparing IgG memory B cell frequencies follo ...
Broad Serum Neutralization of N276A Viruses Following immunization, purified serum IgG from each of the 12 mice receiving the en ...
Figure 3. Genetic Maturation of Immunogen-Induced Anti- bodies (A) Frequency of VRC01-class (VH1-2 heavy chain and a 5AA long LC ...
described earlier. Generally, neutralization breadth of mAbs from each mouse correlated with the breadth of serum neutralization ...
substantially more potent against isolates lacking the N276 glycan, but removal of the N276 glycan site does not make tier 2 vir ...
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