Cell - 8 September 2016
case of a solvent of roughly equal molecular volume, e.g., the protein environment in the cell. For largen,ccx 0 : 5 +n^1 =^2. ...
wheresMR+;andsPR+;are the gain(+), loss(-) terms for reaction pathways, Equation 7 and Equation 11 , respectively. Compatibili ...
Supplemental Figures (legend on next page) ...
Figure S1. Characterization of PGL-3 Drop Properties in the Presence or Absence of mRNA, Related toFigures 1and 3 (A and B) Maxi ...
Figure S2. Contribution of Different Structural Features of RNA to PGL-3 Drop Assembly, Related toFigure 3 (A–D) Maximum intensi ...
Figure S3. Example smFISH and RNA-Seq Analysis to Quantify mRNA inC.elegansEmbryos, Related toFigure 2 (A) Example smFISH and RN ...
Figure S4. Characterization of the Inhibition of MEX-5 on mRNA-Dependent PGL-3 Drop Assembly, Related toFigure 4 (A) Effect of M ...
Figure S5. Characterization of Binding between PGL-3 andC.elegansRNA, Related toFigure 5 (A–C) qPCR analysis of RNA that co-immu ...
(legend on next page) ...
Figure S6. Theoretical Model of the Influence of MEX-5 on Phase Separation of PGL-3 and mRNA, Related toFigures 3, 4 , and 6 (A) ...
Resource Development of a Comprehensive Genotype-to- Fitness Map of Adaptation-Driving Mutations in Yeast Graphical Abstract Hig ...
Resource Development of a Comprehensive Genotype-to-Fitness Map of Adaptation-Driving Mutations in Yeast Sandeep Venkataram,1,7B ...
high-frequency and easy to sequence mutations. Moreover, separating the adaptive mutations from those that are merely hitchhikin ...
from replicate E2. Those evolutions were performed by serial transfer in limiting glucose conditions, such that the populations ...
(0% <s< 5%). To understand the spectrum of neutral mutations, we also sequenced 85 neutral clones. Our sequenced clones th ...
Table 1. Summary of Mutations Observed in this StudyClass Locus No. ofClones No. of Clones withNo MutationsOutside Locus Synonym ...
MATastrain (the mating type of the founding ancestor). Thus, all of the diploids apparently arose via self-diploidization to gen ...
We also note that four diploid clones (not included in the 82 described above) also carried mutations in the nutrient response p ...
variation among the lineages with mutations inGPB2demon- strates the precision of our fitness estimates and further sug- gests t ...
(s3.4%), and (2) mutations presumably activating the Ras/ PKA or TOR/Sch9 pathways (s5%–15%). These two classes of mutations e ...
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