Cell - 8 September 2016
Baumli, S., Hoeppner, S., and Cramer, P. (2005). A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Sr ...
Robinson, P.J., Trnka, M.J., Pellarin, R., Greenberg, C.H., Bushnell, D.A., Davis, R., Burlingame, A.L., Sali, A., and Kornberg, ...
STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins 3-(Decyldim ...
CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagents may be directed to the corresponding auth ...
EXPERIMENTAL MODEL AND SUBJECT DETAILS Saccharomyces cerevisiae The endogenous yeast transcription proteins used in Med-PIC asse ...
reference projection image and micrograph were normalized to mean = 0, SD = 1. Automatic picking was performed using a corre- la ...
free for SBAT cross-linking. Formaldehyde pre-treatment proceeded for 10–20 min on ice after which SBAT was added identically to ...
harboring a exogenous WTRPB1gene copy within the pRS316 yeast centromeric vector (URA+)(kind gift of Craig Kaplan, Texas A&M ...
Satisfaction of the EM restraint is scored using the integral of the ‘‘overlap function’’ between GMMs calculated for EM map and ...
QUANTIFICATION AND STATISTICAL ANALYSIS Statistical confidence in the assignment of cross-linked spectra was determined using a ...
Supplemental Figures Figure S1. Cryo-EM Raw Data and Reference Structures, Related toFigure 2 (A) Med-PIC cryo-electron tomograp ...
Figure S2. Med-PIC Targeted Refinement Strategy, Related toFigure 2 (A) Heterogeneity analysis with high-dose/defocus particle s ...
Figure S3. Cross-linking Mass Spectrometry Datasets from Current and Published Studies, Related toFigure 3 (A) Network diagrams ...
Figure S4. Med-PIC Modeling, Related toFigure 2 (A) Docking of RNA pol II crystal structure shown in the canonical front view. ( ...
Figure S5. Mediator-Pol II Interaction Studies, Related toFigure 5 (A) Surface plasmon resonance (SPR) curves for the interactio ...
Figure S6. Localizing TFIIF Subunits with Integrative Modeling and Cross-linking, Related toFigure 2 (A) Localizing TFIIF subuni ...
Figure S7. Resolving TFIIK and Mediator Tail density, Related toFigures 6and 7 (A) Comparison and superposition of the 2.5 MDa M ...
Article A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes Graphical Abstract Highlights d CRISPR ...
Article A Genome-wide CRISPR Screen inToxoplasma Identifies Essential Apicomplexan Genes Saima M. Sidik,1,7Diego Huet,1,7Suresh ...
SeveralstudieshavedevelopedCRISPR/Cas9-basedgenome- wide genetic screens for mammalian cells (Koike-Yusa et al., 2014; Shalem et ...
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