Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)
Engineered Ionizable Side Chains 13 protonated, this finding is entirely consistent with the high pKa value of the gua- nidine g ...
14 G. D. Cymes and C. Grosman inward rectification; three clear examples of this phenomenon are the lysine mu- tants at position ...
Engineered Ionizable Side Chains 15 the transmembrane pore proper). In fact, the latter may well be the reason why ly- sines eng ...
16 G. D. Cymes and C. Grosman and/or deprotonated open-channel dwell times may follow exponential distributions with more than a ...
Engineered Ionizable Side Chains 17 we reported pertain to those estimated from the bursts of long openings. As for the number o ...
18 G. D. Cymes and C. Grosman does, and hence, its pKa need not remain the same. This phenomenon, which is not predicted by the ...
Engineered Ionizable Side Chains 19 pH? What pH? Once the ratio of occupancies of the deprotonated and protonated forms of an en ...
20 G. D. Cymes and C. Grosman Although recording from excised patches would have allowed us to use solutions of the same pH on e ...
Engineered Ionizable Side Chains 21 receptor, for example, or even a phenomenological quantity such as an EC 50 value, it is the ...
22 G. D. Cymes and C. Grosman to become slower when deuterium oxide replaces water as the solvent because of the known effect of ...
Engineered Ionizable Side Chains 23 Liao M, Cao E, Julius D, Cheng Y (2013) Structure of the TRPV1 ion channel determined by ele ...
25 M. H. Akabas () Departments of Physiology & Biophysics, Neuroscience and Medicine, Albert Einstein College of Medicine, ...
26 M. H. Akabas Keywords Sulfhydryl · Thiol · Thiolate · SCAM · GABAA receptor · Acetylcholine receptor · Potassium channels · M ...
Cysteine Modification 27 local environment can significantly alter the cysteine pKa (Jones et al. 1975 ; Shaked et al. 1980 ; Ho ...
28 M. H. Akabas 2000 ; Riegelhaupt et al. 2010 ) and receptors (Javitch et al. 1995 ), in their native lipid membrane environmen ...
Cysteine Modification 29 and screening of a large number of mutants and functional characterization of the mutants. We have foun ...
30 M. H. Akabas 1996 ; Liu et al. 1996 ; Larsson et al. 1996 ; Yamagishi et al. 1997 ; Beck et al. 1999 ; Boileau et al. 1999 ; ...
Cysteine Modification 31 can alter reaction rates by several orders of magnitude (Bali and Akabas 2012 ). Comparison of MTS reag ...
32 M. H. Akabas (Akabas et al. 1992 ; Karlin and Akabas 1998 ). One can factor out the influence of the electrostatic interactio ...
Cysteine Modification 33 2.3 Maleimides Maleimides have an advantage over MTS reagents and mercurials in that they form a thioet ...
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