Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)
34 M. H. Akabas their reaction rates increase at higher pH (Gorin et al. 1966 ). A disadvantage of the maleimides is, however, t ...
Cysteine Modification 35 ligand-gated ion channels (pLGIC). After the cloning of the nicotinic acetylcholine receptor subunits ( ...
36 M. H. Akabas identified by this approach are a subset of those identified by SCAM studies pro- viding validation for both app ...
Cysteine Modification 37 tor (Cymes et al. 2005 ). Crystal structures have been solved for several Cys-loop receptor homologues ...
38 M. H. Akabas the cation-selective subunits and an alanine in the anion selective subunits. A recent paper studying muscle-typ ...
Cysteine Modification 39 the electrostatic potential in the binding site. They measured the effect of reagent charge and ionic s ...
40 M. H. Akabas Conformational Change During Gating and Gate Location Another powerful use of SCAM is to detect channel regions ...
Cysteine Modification 41 lar solution, it was concluded that the closed channel gate was at the cytoplasmic end of the channel. ...
42 M. H. Akabas tracellular end of M3. Surprisingly, in the presence of GABA, cysteines at multiple positions along the extracel ...
Cysteine Modification 43 3.2 A Few Examples of SCAM in Other Channels Extensive SCAM studies have been done in other ion channel ...
44 M. H. Akabas 4 Conclusions Cysteine substitution mutagenesis has been a powerful approach for studies of the structure and fu ...
Cysteine Modification 45 Beck C, Wollmuth LP, Seeburg PH, Sakmann B, Kuner T (1999) NMDAR channel segments forming the extracell ...
46 M. H. Akabas Cha A, Bezanilla F (1997) Characterizing voltage-dependent conformational changes in the Shak- er K+ channel wit ...
Cysteine Modification 47 Dodier Y, Banderali U, Klein H, Topalak O, Dafi O, Simoes M, Bernatchez G, Sauve R, Parent L (2004) Out ...
48 M. H. Akabas Gkountelias K, Papadokostaki M, Javitch JA, Liapakis G (2010) Exploring the binding site crevice of a family B G ...
Cysteine Modification 49 Imoto K, Methfessel C, Sakmann B, Mishina M, Mori Y, Konno T, Fukuda K, Kurasaki M, Bujo H, Fujita Y et ...
50 M. H. Akabas Kluger R, Tsui WC (1980) Amino group reactions of the sulfhydryl reagent methyl methanesul- fonothioate. Inactiv ...
Cysteine Modification 51 Lu J, Deutsch C (2005) Secondary structure formation of a transmembrane segment in Kv chan- nels. Bioch ...
52 M. H. Akabas teine accessibility. J Biol Chem 286(16):14098–14109. doi:M111.221895 [pii] 10.1074/jbc. M111.221895 Pascual JM, ...
Cysteine Modification 53 Slatin SL, Qiu XQ, Jakes KS, Finkelstein A (1994) Identification of a translocated protein segment in a ...
«
1
2
3
4
5
6
7
8
»
Free download pdf