Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)
54 M. H. Akabas Williams DB, Akabas MH (2002) Structural evidence that propofol stabilizes different GABA(A) receptor states at ...
55 F. Bezanilla () · M. Priest Department of Biochemistry and Molecular Biology and Committee on Neurobiology, University of Ch ...
56 M. Priest and F. Bezanilla 1 Introduction Fluorescence has long been used in biology to follow molecular motions in proteins. ...
Functional Site-Directed Fluorometry 57 to the development of site-directed fluorometry, examination of conformational changes i ...
58 M. Priest and F. Bezanilla clamp, with no need for protein purification and reconstitution ‒ with those of site- labeled spec ...
Functional Site-Directed Fluorometry 59 gene of interest. Site-directed mutagenesis of the gene should be done to ensure that on ...
60 M. Priest and F. Bezanilla Fig. 1 Basic steps in functional site-directed fluorometry. Schematic drawings not to scale. a Mol ...
Functional Site-Directed Fluorometry 61 Next, a single labeled oocyte is placed in the electrophysiological recording chamber an ...
62 M. Priest and F. Bezanilla significantly in the red region of the spectrum so red fluorophores may provide a better signal-to ...
Functional Site-Directed Fluorometry 63 (Cha et al. 1999b; Chanda et al. 2005 ; Glauner et al. 1999 ; Hyde et al. 2012 ; Posson ...
64 M. Priest and F. Bezanilla behave fundamentally similarly to those in more typical channels (Sesti et al. 2003 ). Other work ...
Functional Site-Directed Fluorometry 65 more frequently, inter-molecular interactions. An excellent example is how site- directe ...
66 M. Priest and F. Bezanilla Importantly, conformational changes measured by fluorometry can be wholly induced by small molecul ...
Functional Site-Directed Fluorometry 67 Like the investigations of cooperativity between subunits in voltage-gated ion channels, ...
68 M. Priest and F. Bezanilla gating current might not be is because the fluorescence tends to follow the gating charge, which i ...
Functional Site-Directed Fluorometry 69 fluorophore fluorescence to comprise the total fluorescence change observed during chann ...
70 M. Priest and F. Bezanilla 4 Outlook Functional site-directed fluorometry is a powerful tool for investigating in vivo con- f ...
Functional Site-Directed Fluorometry 71 Blunck R, Starace DM Correa AM, Bezanilla F (2004) Detecting rearrangements of Shaker an ...
72 M. Priest and F. Bezanilla Dekel N, Priest MF, Parnas H, Parnas I, Bezanilla F (2012) Depolarization induces a conforma- tion ...
Functional Site-Directed Fluorometry 73 Khatri A, Sedelnikova A, Weiss DS (2009) Structural rearrangements in loop F of the GABA ...
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