Nucleic Acids in Chemistry and Biology
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Glossary AGAROSE: A polysaccharide isolated from seaweed used as a matrix in gel electrophoresis. ALLELE: One of two alternate f ...
BACK MUTATION: Reverses the effect of a mutation that had inactivated a gene. BACTERIOPHAGE: A virus that infects bacteria; ofte ...
COSMIDS: Plasmids into which phage lambda cos sites have been inserted; as a result, the plasmid DNA can be packaged in vitroint ...
EXONUCLEASE: An enzyme that cleaves nucleotides one at a time from the end of a polynucleotide chain. Such enzymes may be specif ...
HYBRIDIZATION: The pairing of complementary RNA and DNA strands to give an RNA–DNA hybrid. It is also used to describe the pairi ...
LINKER(FRAGMENT): A short synthetic duplex oligonucleotide containing the target site for some restric- tion enzyme. A linker ma ...
OLIGOMER: Term often used in place of oligonucleotide. OLIGONUCLEOTIDE: Polymer comprising of nucleotide units (usually less tha ...
PRIMER: A short sequence (of DNA or RNA) that is paired with one strand of DNA and provides a free 3 -OH end at which a DNA pol ...
REVERSE TRANSCRIPTASE: RNA-dependent DNA polymerase. Originally detected in retroviruses. It is, how- ever, also present in norm ...
SIGNAL TRANSDUCTION: Molecular mechanism of transferring the information from the outside of a cell, a receptor, to the nucleus. ...
TRANSDUCTION: The transfer of a bacterial gene from one bacterium to another by a phage; phage carry- ing host as well as its ow ...
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CHAPTER 1 Introduction and Overview CONTENTS 1.1 The Biological Importance of DNA 1 1.2 The Origins of Nucleic Acids Research 2 ...
turned a searchlight on the molecular nature of nucleic acids and it soon became evident that ideas on the chemistry of nucleic ...
Thymus nucleic acid, which was readily available from calf tissue, was found to be resistant to alkaline hydrolysis. It was only ...
The scientists compromised. In his Tilden Lecture of 1943, Masson Gulland suggested that the concept of nucleic acid structures ...
Introduction and Overview 5 N N N N NH 2 O HO P O OH HO O N N N N OH O HO P O OH HO O NH 2 deoxyadenylic acid [dAMP] deoxycytidy ...
from stretched, dry fibres of DNA. From the rather obscure data he deduced ‘... A spacing of 3.34 Å along the fibre axis corresp ...
On the basis of the advice from Jerry Donohue in the Cavendish Laboratory, Watson turned to manipulating models of the bases in ...
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