Multiphase Bioreactor Design

whether a cell will adsorb to an air-bubble. Adsorption only occurs if the change in free energy upon adsorption is negative. Th ...

Table 15.5 Minimal contact time, τ, for cell attachment to air bubbles cell movement and liquid drainage, and half life (t0.5) a ...

where μ is the dynamic viscosity, and dv/dx is the velocity gradient. For the velocity gradient dv is given by the maximum injec ...

given by Kioukia et al. (1992), who suggest that, although the mean bubble diameter was equal in both situations, the distributi ...

according to this theory the killing volume would be proportional to the square of the bubble diameter, as is the case for the m ...

Figure 15.3 Schematic drawing of the projected radius for a rising bubble (Adapted from Meier et al. (1999). where τ(s) is the m ...

Figure 15.4 Rise-dependent killing volume as a function of: A) bubble diameter and B) reactor height. Required contact times for ...

for a 3.5 mm bubble (van der Pol et al. 1992), the contribution by the rise-dependent killing-volume would become significant on ...

higher than one meter, while in the presence of these additives the adsorption of cells to bubbles is minimal and the height dep ...

Figure 15.5 Schematic drawing of the bubble burst process. Arrows on the left connecting b and e show the origin of the liquid p ...

The entrapment and drainage of cells from a bubble film was studied by Michaels et al. (1995a) by microscopically looking at ind ...

(21) where Rb (m) is the bubble radius. The rate of energy dissipation per unit mass for a surface tension of 64 mN·m−^2 , a hem ...

exponentially when the bubble diameter increases from 0.77 to 6.3 mm. This is in accordance with the finding of Handa et al. (19 ...

by 75%, meaning that about 900 cells were killed per bubble burst. This is somewhat lower than the value of 1150 cells found pre ...

presence of Pluronic. However, in the presence of 0.1% Pluronic more liquid was present in the upward jet and Trinh et al. (1994 ...

stated, the cell concentration in the foam will depend on the attachment of cells to bubbles, the cell concentration, the amount ...

In serum-free medium the separation factor was larger than unity indicating cell- bubble attachment, which is in agreement with ...

Finally, Murhammer and Goochee (1990a) showed that certain small molecular weight Pluronics, like Pluronic L35 and F38 offer jus ...

Michaels et al. (1996) showed that these phenomena did not cause significant cell damage. Finally, Yang and Wang (1992) studied ...

For designing bubble columns and airlift reactors the hypothetical-killing-volume theory of Tramper can be used. The killing vol ...