Systems Biology (Methods in Molecular Biology)

explored key signatures for metastatic prostate cancer. These genes are members of the AP1 transcription factor family. These tr ...

We promote here an integrative workflow that combines net- work structural and dynamical analysis with high-throughput –omics da ...

Lee W-P, Tzou W-S (2009) Computational methods for discovering gene networks from expression data. Brief Bioinform 10:408–423 Z ...

Samaga R, Saez-Rodriguez J, Alexopoulos LG et al (2009) The logic of EGFR/ErbB signaling: theoretical properties and analysis o ...

Kristensen VN, Lingjaerde OC, Russnes HG et al (2014) Principles and methods of inte- grative genomic analyses in cancer. Nat R ...

correlated with downregulation of B-cell lym- phoma-2 and upregulation of microRNA- Oncol Lett 11:817–822 Esber N, Le Billan F, ...

Chapter 13 Spatiotemporal Fluctuation Analysis of Molecular Diffusion Laws in Live-Cell Membranes Francesco Cardarelli Abstract ...

purification, labeling of the molecule of interest with a suitable marker and its re-introduction into the living system; (2) th ...

correlation (Fig.1c). In the presence of diffusion only, the correla- tion function can be interpolated by a Gaussian function ( ...

depends on the dynamics of the molecules moving in the collected image series. In particular, the correlation function is define ...

Phosphate Buffer Solution (PBS) for cell washing procedures. Latrunculin B solution for actin depolymerization. Store at  20  ...

Dilute 10μl of 30 nm fluorescent beads solution (about 5μM) in 90μl of distilled water (seeNote 6). Cut a squared piece (1 cm1 ...

3.3 Data Acquisition 1. Align the TIRF laser excitation according to the procedure of your setup. In the setup proposed here, an ...

from each image its average intensity, as previously demonstrated [24]. Calculate the spatiotemporal correlation (Eq. 1, G(ξ,χ, ...

Fig. 4Analysis of TfR-GFP dynamics in living cells. (a) Schematic representation of a GFP-tagged TfR diffusing within the cytosk ...

confined diffusion in a meshwork and dynamic partitioning into membrane nano-domains (i.e., lipid rafts). In fact, this latter i ...

maintenance Petri dish. Higher accuracy can be reached by counting the detached cells before plating them. The “Cropped Mode” t ...

the Objective numerical aperture and the excitation wave- length). Thus, a pixel size of 70–150 nm (three times lower than the i ...

Low fluorescence intensity cells should be preferred, as the membrane is closer to the native condition and the probability of ...

multiplied CCD camera. Biophys J 95:5385–5398 Di Rienzo C, Gratton E, Beltram F et al (2013) Fast spatiotemporal correlation sp ...