Quorum Sensing

Silicone to assemble the cover slips with the flow chambers (see Note 1). Surface-attached biofilms are cultivated in flow cham ...

2.3 Culture Preparation for DNA Microarray 1.Bacterial strain:P. aeruginosa(PAO1-ATCC) obtained from the Pseudomonas genetic sto ...

3.1 QS Monitor Assays Inoculate the two monitor strains (LasB-GFP or RhlA-GFP) in 2  10 ml ABT media supplemented with 0.5% (w ...

Use minimum two medium flasks. Hook, for example, chan- nels 1–8 up to the medium flask which contains the test QSI compound an ...

The biofilm is investigated after approximately 24 h. For examination of the biofilm with CSLM, a viability staining kit is use ...

3.3 Culture Preparation for DNA Microarray InoculateP. aeruginosa(PAO1) in AB medium supplemented with 0.5% (wt/vol) casamino a ...

Syto 9 and PI are light sensitive and have to be covered with aluminum foil. Remember to also cover the flow cells with aluminu ...

Jakobsen TH, van Gennip M, Phipps RK, Shanmugham MS, Christensen LD, Alhede M et al (2012) Ajoene, a sulfur-rich molecule from ...

Chapter 22 A Coculture-Based Approach for Screening Campaigns Aimed at Identifying NovelPseudomonas aeruginosa Quorum Sensing In ...

antibiotic-resistant strains, novel approaches should be undertaken in order to identify new drugs [1, 2]. An innovative strateg ...

interfering with the activity/stability of the 3OC 12 -HSL receptor LasR or with the functionality of the 3OC 12 -HSL synthase L ...

Automated luminometer-spectrometer plate reader. UV/Vis spectrophotometer. Plastic microcuvettes for UV/Vis spectrophotometer. ...

3.2 Primary Screening Procedure GrowP. aeruginosa PA14 wild type and the 3OC 12 -HSL reporter strain PA14-R3 (seeNote 1) overni ...

molecule, as an electron shuttle for bacterial respiration, and as an antibacterial and antifungal agent [21, 22]. Therefore, a ...

MeasureA 600 and LCPS, simultaneously (seeNote 2). Average theA 600 and LCPS measurements of the replicates. Normalize the aver ...

consider that growth of biosensor strains different from PA14- R3 may require LB supplementation with antibiotics for plas- mid ...

We wish to thanks all the colleagues contributing to the develop- ment of PA14-R3 and to its application in screening campaigns: ...

Ohman DE, Burns RP, Iglewski BH (1980) Corneal infections in mice with toxin A and elastase mutants ofPseudomonas aeruginosa.J ...

Chapter 23 A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin Kaihao Tang and Xia ...

weaken the selective pressure imposed on the pathogens and reduce the potential for evolution of drug resistance [8]. To date, t ...