Quorum Sensing

3.5 Directed Evolution of GKL The generation ofgklmutated genes is obtained by PCR amplifica- tion with a blend of two error-pro ...

Pick colonies that have enhanced quorum-quenching activity relative to theE. coliJLD271 reporter cells expressing wild- type GK ...

limit the amount of homoserine lactone that you may use in the assay. The kinetic assay uses the change in absorbance at 577 nm ...

You may prepare a batch of electrocompetent cells instead of using the commercially prepared cells. Grow overnight at 37 C a 5 ...

Chapter 25 Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones Soumya Palliyil Abstract A numb ...

target specificity and less off-target cytotoxicity [2]. Quorum quenching using antibodies would not only block the signalling c ...

Fig. 1Diagrammatic representation of various steps involved in the construction of an immunized library Monoclonal Antibodies In ...

2 Materials All solutions and media should be sterilized by autoclaving and prepared using water purified to 18.2 MΩ/cm. 2.1 Buf ...

2.2 Culture Media 1. 2
Tryptone-Yeast (TY) medium: 16 g/L tryptone, 10 g/L yeast extract, 5 g/L NaCl. Add 2% (wt/vol) glucose an ...

with the phagemidoriof replication. It also carries a gene for Km resistance to aid selection of infected bacteria. Wild-typeCa ...

isopropylβ-D-thio galactosidase (IPTG) and contains a human Cκdomain downstream of the cloned antibody fragment that facilitates ...

Ovine lambda chain variable region 5^0 primers. (a) OvVL1LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC GAG TCT AAA GTG GAT GAC CAG GCT ...

(c) MuVH1BACK-Sfi:5^0 -CAT GCC ATG ACT GCG GCC CAG CCG GCC ATG GCC SAG GTS MAR CTG CAG SAG TCW GG-3^0. (d) Hu4aBACK-Sfi:5^0 -GTC ...

Quantification of sheep polyclonal IgG concentration by Cap- ture ELISA. A 96-well flat bottomed ELISA plate is incubated with ...

Indirect competition ELISA. 100μL aliquots of PBS contain- ing 1μg/mL AHL-TG conjugates are dispensed in a polysty- rene 96-wel ...

Grow the bacterial cultures at 37C with shaking at 200 rpm until an A 600 ffi2.0. Record the A 600 of the bacterial cultures a ...

The plates containing young worms are washed with M9 buffer, transferred onto NGM agar plates containing fresh lawns ofE. coliO ...

Approximately 30 mL sheep blood is carefully poured onto each Histopaque-1077 column and spun at 2000
g for 15 min at room tem ...

(c) MuVH1BACK and mixture of OvJH1LINKFOR, OvJH2- LINKFOR, OvJH3LINKFOR, OvJH4LINKFOR. (d) Hu4aBACK and mixture of OvJH1LINKFOR, ...

electrophoresed at approximately 1 V/cm^2 , using TAE buffer, until the dye front reach three-fourth of the agarose gel. The DNA ...