Quorum Sensing

4 Notes Never add glucose to the medium because glucose inhibits bioluminescence ofVibrio harveyi. The chance of identifying tr ...

(1981) Structural identification of autoinducer ofPhotobacterium fischeriluciferase. Biochem- istry 20:2444–2449 Henke JM, Bass ...

Chapter 27 Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of o ̃-Hemolysin with High Performance L ...

screening efforts [3–7]. o ̃-hemolysin is a direct output of theagr system and thus serves an ideal product to track for the dis ...

Mobile Phase B: 0.1% (vol/vol) TFA in acetonitrile (ACN). HPLC system. 2 ml HPLC vials. 3 Methods Carry out all experiments at ...

3.Day 3: Standardize the inoculum to a final starting density of 5  105 colony forming units (CFU) per ml by diluting into fres ...

9.Day 4: Remove cultures from the incubator and immediately place tubes on ice. Take a 100μl aliquot of each culture and transfe ...

The chromatogram will show two prominent peaks, one corresponding to the deformylated o ̃-hemolysin at a retention time of ~7.2 ...

hemolysin must be divided by the OD reading determined in Subheading3.2,step 9 Anorm¼ Araw OD 600 Once the integration data has ...

Example: To achieve a starting concentration of 32μg/ml, using a drug stock concentration of 10 mg/ml and a starting volume of ...

INDEX A Accessory gene regulator (Agr)..............89–95, 179, 363 Actinorhodin................................................ ...

Coenzyme A (CoA)................................................ 36, 37, 162–165, 167–171, 173, 174 Conformational change....... ...

Xylella fastidiosa............................................... 97, 243 Gram-positive bacteria Bacillus subtilis.............. ...

N N-acyl-homoserine (HS) .................................... 4,61–67, 69–71, 203–211, 297, 307, 311 Next-generation sequencing ...

LuxN............................................................74, 355 LuxO.................................................262 ...