Quorum Sensing
to 98C for 30 s and subjected to two cycles of annealing and polymerization using 65C as the annealing temperature for VH-Vλan ...
Single colonies fromstep 12are picked from serial dilution plates of VH-Vλand VH-Vкlibraries and used as source of template DNA ...
After overnight incubation at 30C, the number of plaques on each dilution plate is counted and helper phage titer calculated a ...
2 mL PBS, spin for 10 min at 17,000gon a tabletop centrifuge to remove any bacterial debris. The phage obtained after double PE ...
3 oxo C12 BSA (50 ug/ml) 3oxo C12 BSA (100 ng/ml) 3 oxo C12 BSA (100 ng/ml) TEA Elution (100 mM) TEA Elution (100 mM) TEA Elutio ...
to the AHL-conjugate in the immunotube and washing as described in Subheading3.6,step 13, 4 mL of free AHL at a desired concentr ...
immunotube coated with AHL-conjugate as described insteps 11 – 17. Subsequent rounds of rescue and selection are carried out and ...
overnight at 30 C with shaking. Centrifuge the plate at 700 gfor 10 min and transfer the supernatant containing monoclonal pha ...
Dialyze the ligation mix on a 0.025μm filter paper against sterile water for 30 min and transform into electrocompetent XL1-Blu ...
recover the supernatant containing released protein, and filter through a 0.30μm filter. For purification of scAbs, IMAC Sephar ...
purified antibody fragments to the wells alongside double dilu- tions of human whole IgG (кisotype) with starting concentra- tio ...
physiological pH and the antibody present in the flow-through is collected and used for downstream purposes without any need for ...
Chapter 26 Identification of AI-2 Quorum Sensing Inhibitors inVibrio harveyiThrough Structure-Based Virtual Screening Tianyu Jia ...
molecules able to interfere with AI-2 QS could lead to the develop- ment of next generation broad-spectrum antimicrobial agents ...
a switch from kinase to phosphatase activity, which results in dephosphorylation of the downstream proteins LuxU and LuxO. Depho ...
2 Materials Virtual screening (DOCK 6) was performed using a Linux- based cluster. Visualization (PyMOL 0.99, HBPLUS 3.06, and ...
3 Methods 3.1 Virtual Screening 1. The database including 0.5 million compounds from Specs Ltd. is first converted into 3D struc ...
Check the hydrogen bond and hydrophobic interaction pro- files of the selected top 10% compounds by the IDEA 8.8 software (IDEA ...
supplemented with 50 μg/mL kanamycin and 10 μg/mL chloramphenicol. The culture is grown for 16 h with aeration (175 rpm) at 30C ...
3.4 Effect of the Test Compounds on Bacterial Growth Rate A single colony ofV. harveyiMM32 is picked from the LM plate prepared ...
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