Quorum Sensing
micromolar concentrations. The third section describes theC. violaceumAHL bioassay for detecting C4- and C6-HSLs on the basis of ...
1.4 Biofilms and Biofilm Dispersion Adhesion to and growth on surfaces as biofilms is a common feature of bacteria and other mic ...
tolerance, since microorganisms regain planktonic levels of suscep- tibility once they leave the biofilm community [43]. Just as ...
While intracellular c-di-GMP concentrations become elevated in biofilms via diguanylate cyclases, the levels decrease notably in ...
Bath sonicator (for environmental biofilm samples): We have found it important to use glass vials rather than plastic as the la ...
4.A. tumefaciens KYC6 (pCF218)(traM-tn5-gusA,KmR) (3-o-C8-HSL overproducing strain used as positive control forA. tumefaciensAHL ...
2.3 Chromo- bacterium violaceum AHL Detection (See Note 1) 1.C. violaceumCVO26 (biosensor strain) [11]. 2.C. violaceumATCC 31532 ...
Microtiter plate reader (preferably with temperature control and ability to agitate samples). Multichannel pipets are very usef ...
exposure to the bioassay organism. Although antibiotics are required to maintain the plasmids in strains A136 (pCF218) (pCF372) ...
7.Day Three: Examine and photograph plates. A positive result is indicated by a loss of pigmentation in the vicinity (seeNotre 6 ...
Normally these two strains will cross-feed each other (positive control for AHL detection) such that strain CVO26 will produce v ...
spread evenly over TLC plate). Immediately pour mixture over pre-warmed plate and spread evenly to cover. Layer should be approx ...
Add 200μl of candidate dispersant solution (a buffer such as PBS can be used as a negative control). Place in plate reader (if ...
employlacZas a reporter gene and require supplementation of X-gal to the medium. A comparison of the sensitivity of theC. violac ...
Schertzer JW, Boulette ML, Whiteley M (2009) More than a signal: non-signaling properties of quorum sensing molecules. Trends M ...
Fuqua C, Winans SC (1996) Conserved cis- acting promoter elements are required for density-dependent transcription ofAgrobacter ...
McLean RJC, Whiteley M, Stickler DJ, Fuqua WC (1997) Evidence of autoinducer activity in naturally-occurring biofilms. FEMS Mic ...
Chapter 2 Detection of 2-Alkyl-4-Quinolones Using Biosensors Matthew P. Fletcher, Stephen P. Diggle, Miguel Ca ́mara, and Paul W ...
rhamnolipids, pyocyanin, lectins, superoxide dismutases, and bio- film development [1]. Pesci et al. [2] demonstrated that addit ...
For the detection and unequivocal chemical identification of AQs, both NMR (Nuclear Magnetic Resonance) spectroscopy and LC-MS ( ...
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