Organ Regeneration Based on Developmental Biology

© Springer Nature Singapore Pte Ltd. 2017 193 T. Tsuji (ed.), Organ Regeneration Based on Developmental Biology, DOI 10.1007/978 ...

194 Abbreviations BMP Bone morphogenetic protein CM Cap mesenchyme EE Embryonic ectoderm FGF Fibroblast growth factor GBM Glomer ...

195 accelerated by acute or chronic kidney diseases, and while tubular epithelial cells can respond to elicit repair following m ...

196 11.1.1 Formation of the Mammalian Kidney Studies into the development of the mammalian kidney, largely performed in mouse, h ...

197 mesoderm most closely associated with the tips, referred to as the cap mesenchyme (CM). This feedback loop restricts the dom ...

198 defined culture conditions reported to be sufficient to maintain a CM phenotype in vitro away from the niche (Brown et al. 2 ...

199 11.2.1 Induction of the Primitive Streak During Development and In Vitro The generation of both endodermal and mesodermal ti ...

200 11.2.2 Inducing the Intermediate Mesoderm During Development and In Vitro The trunk mesoderm of the vertebrate embryo is for ...

201 the duration of CHIR99021-mediated specification between 2 and 5 days before the addition of FGF9 (Takasato et al. 2015 ). A ...

202 facilitate spontaneous self-organisation and potentially improve morphogenesis in vitro. In a protocol using fully defined A ...

203 11.3 Alternative Protocols for Directing Differentiation of hPSCs to the Kidney Alongside the identification of this protoco ...

204 differentiation of human iPSC to a single renal cell type or segment (Song et  al. 2012 ; Wang et al. 2013 ; Sharmin et al. ...

205 Author,Year CultureMethod Derivation of IntermediateMesoderm (IM) via PosteriorPrimitive Streak (PPS) Derivation of Metaneph ...

206 spheroids from iPSC and then induced epithelial-to-mesenchymal transition using a short period of high-dose CHIR treatment ( ...

207 access to viable material is limited, particularly from late gestation, and genetic tools available in mouse such as lineage ...

208 11.4.2 Drug Screening Using Kidney Organoids Drug toxicity is a major cause of attrition in late-stage clinical trials and o ...

209 Organoids transplanted under the renal capsule of immunocompromised mice or rats show host-derived vascularisation in their ...

210 establish a structural or molecular phenotype. Freedman et  al. ( 2013 ) identified lower ciliary expression of polycystin-2 ...

211 opportunities for clinical trials in the treatment of Parkinson’s disease (Barker et al. 2012 ), and the generation of retin ...

212 reintroduced to a patient and functionally contribute. Two such studies have been reported. The first of these (Imberti et a ...