Organ Regeneration Based on Developmental Biology

29 2.2 Self-Organization of Cerebellar Tissue from hPSCs in 3D Culture 2.2.1 Development of Mammalian Cerebellum In humans, the ...

30 be intersected by as many as 200,000 parallel fibers (Braitenberg and Atwood 1958 ). Purkinje cell dysfunction (hereditary or ...

31 At first, cerebellar differentiation from mouse ESCs (mESC) has accomplished a combinatory treatment of positional signals (S ...

32 efficiency of Purkinje cell differentiation, it requires a large amount of growth factors, and it does not appear to induce t ...

33 signals Fgf8 and Wnt1. The self-formation of isthmic organizer tissue in this culture conditions is also dependent on Fgf8 an ...

34 2.3 Construction of Human Cerebellar Disease Models with Patient-Derived iPSCs Construction of neuronal disease models with h ...

35 Fig. 2.6 Self-formation of stratified cerebellar neuroepithelial structure. (a–d) Immunostaining of Fgf19-treated aggregates ...

36 Li et al. 2005 , 2008 ; Patani et al. 2011 ; Perrier et al. 2004 ; Perrier and Peschanski 2012 ; Sánchez-Danés et al. 2012 ; ...

37 and selective vulnerability. The self-organizing culture technique that differentiates hPSCs into cerebellar neurons will bec ...

38 Gaspard N, Bouschet T, Hourez R et  al (2008) An intrinsic mechanism of corticogenesis from embryonic stem cells. Nature 455: ...

39 Mizuseki K, Sakamoto T, Watanabe K et al (2003) Generation of neural crest-derived peripheral neurons and floor plate cells f ...

40 Wataya T, Ando S, Muguruma K et al (2008) Minimization of exogenous signals in ES cell culture induces rostral hypothalamic d ...

© Springer Nature Singapore Pte Ltd. 2017 41 T. Tsuji (ed.), Organ Regeneration Based on Developmental Biology, DOI 10.1007/978- ...

42 Our latest study aimed to prepare hypothalamic and pituitary tissues from human pluripotent stem cells and establish effectiv ...

43 stress- induced changes of hormone requirements. For example, we have reported that some patients with central diabetes insip ...

44 process. During the early development, the adenohypophysis anlage originates as a placode in the nonneural ectoderm adjacent ...

45 3.5 Three-Dimensional ES Cell Culture Organ formation during embryogenesis consists of complicated processes that involve var ...

46 Thus, in the absence of exogenous growth factors in the medium, ES cell-derived neuroectodermal cells spontaneously different ...

47 Koehler et al. succeeded in differentiating the otic placode (Fig. 3.1a) (Koehler et al. 2013 ), which belongs to the head an ...

48 structures formed in the SAG-treated LCA SFEBq aggregates (Fig. 3.2c). The ves- icles were situated between the oral ectoderm ...