AMPK Methods and Protocols

the control and treated tissue will likely show the same degree of AMPK activation. Depending on whether the protein of interes ...

F890–F900. https://doi.org/10.1152/ ajprenal.00308.2016 Pastor-Soler NM, Hallows KR (2012) AMP-activated protein kinase regulat ...

Chapter 29 A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress Se ́bastien Denanglaire, Tiphe` ...

where infiltrating tumor-specific lymphocytes may face severe hyp- oxia and/or reduced glucose availability [10–13]. Studies on ...

further explain the cytometry analysis procedure and viable cell calculation for T lymphocytes identified by flow cytometry (Fig ...

Phosphate-buffered saline (PBS): 145 mM NaCl, 2.7 mM KCl, 1.5 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 ,pH7. Flow medium: PBS, 0.1% (w/v) ...

2.2 Reagents and Materials Dimethyl sulfoxide (DMSO). Oligomycin stock solution: 100μM oligomycin in DMSO. Prepare a 10 mM olig ...

solution in culture medium by adding 10μL of 10 mM stock oligomycin into 990 μL of culture medium (this 100-fold dilution leads ...

(c) Transfer 250μL of cells from tube 1 to tube 2, mix well, and transfer again 250μL of twofold diluted cell suspen- sion from ...

Centrifuge the tubes at 400gfor 5–10 min at 4C. Prepare a “master mix” cell staining solution for lymphocyte subpopulation ce ...

Recovered cells per 1000 beads¼ 1000 ðÞnb of events in viable lymphocyte gate %T cell subset Number of events in bead gate The ...

the 1.5 mL tube by pipetting up and down before taking a few microliters. Be careful of the instrument threshold settings. Bead ...

The plot FSC-H vs. FSC-A is used to discriminate doublets from single cells. In this plot, all events falling outside the diago ...

by capillary electrophoresis time-of-flight mass spectrometry. Cancer Res 69:4918–4925 Ho P-C, Bihuniak JD, Macintyre AN, Staro ...

Chapter 30 Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis Nicholas D. LeBlond and Morgan D. Fullerton ...

innate immune cells, hence furthering a pro-inflammatory plaque microenvironment [5, 6]. Macrophages are dynamic innate immune c ...

had the protective effect of inhibiting foam cell formation through the induction of cholesterol efflux [23], suggesting a prote ...

BMDM medium: DMEM containing 4.5 g/L glucose and 1 mM sodium pyruvate, 10% (v/v) heat-inactivated FBS, 100 units/mL penicillin, ...

2.3 Acetylated Low-Density Lipoprotein (acLDL) Uptake Cells of interest: BMDM. ClassІІbiological safety cabinet (BSCІІ). Bead b ...

Aluminum foil. 1.5 mL microfuge tube. Fluorometric plate reader (e.g., Biotek Synergy H1 plate reader). Microplate for fluoresc ...