Synthetic Biology Parts, Devices and Applications
References 231 31 Haft, R.J. et al. (2006) General mutagenesis of F plasmid TraI reveals its role in conjugative regulation. J. ...
232 11 Small Functional Peptides and Their Application in Superfunctionalizing Proteins 51 Baruah, H. et al. (2008) An engineere ...
References 233 71 Butz, M. et al. (2011) An N‐terminal protein degradation tag enables robust selection of highly active enzymes ...
234 11 Small Functional Peptides and Their Application in Superfunctionalizing Proteins 91 Fayolle, C. et al. (1999) Therapy of ...
References 235 108 Brogden, K.A. et al. (1996) Isolation of an ovine pulmonary surfactant‐ associated anionic peptide bactericid ...
237 Parts and Devices Supporting Spatial Engineering Part III ...
239 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...
240 12 Metabolic Channeling Using DNA as a Scaffold and organelle targeting more closely imitate the formation of natural enzyme ...
12.1 Introduction 241 Niemeyer and coworkers [13] were the first to in vitro assemble enzymes on a DNA scaffold. They arranged N ...
242 12 Metabolic Channeling Using DNA as a Scaffold anchoring of the DNA‐binding protein to the DNA‐target site is well characte ...
Table 12.2 DNA scaffolds used to order enzymes of biosynthetic pathways. Product Enzyme DNA‐binding protein and its target site ...
Table 12.2 (Continued) Product Enzyme DNA‐binding protein and its target site Chimeric protein DNA scaffold a) Comments Referenc ...
12.2 Biosynthetic Applications of DNA Scaffold 245 and with two copies of the TS ([1 : 1 : 2] 8 bp), reduced the production time ...
246 12 Metabolic Channeling Using DNA as a Scaffold 12.2.3 1,2‐Propanediol A biosynthetic pathway for 1,2‐propenediol composed o ...
12.3 Design of DNA‐Binding roteins and Target Sites 247 hydroxymethylglutaryl‐CoA reductase (HMGR). The biosynthesis of meva lo ...
248 12 Metabolic Channeling Using DNA as a Scaffold arrangement of the DNA‐binding proteins along the DNA (Figure 12.1d). The ma ...
12.3 Design of DNA‐Binding roteins and Target Sites 249 Taken together, the described results indicate that (i) zinc fingers ret ...
250 12 Metabolic Channeling Using DNA as a Scaffold DNA‐binding sequences in the host genome [29]. The binding affinity between ...
12.4 DNA rogram 251 spacer of four to five nucleotides positions the neighboring two functional domains to the opposite sides of ...
252 12 Metabolic Channeling Using DNA as a Scaffold the DNA program, in which individual DNA‐target sites were separated with sp ...
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