Synthetic Biology Parts, Devices and Applications

274 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 46 Severcan, I., Geary, C., Chworos, A., Voss, N., Jacovetty, E. ...

References 275 64 Win, M.N. and Smolke, C.D. (2007) A modular and extensible RNA‐based gene‐regulatory platform for engineering ...

276 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 81 Ponchon, L. and Dardel, F. (2007) Recombinant RNA technology: ...

References 277 97 Delebecque, C.J., Silver, P.A., and Lindner, A.B. (2012) Designing and using RNA scaffolds to assemble protein ...

278 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 115 Wu, C.‐H., Lockett, M.R., and Smith, L.M. (2012) RNA‐mediate ...

279 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...

280 14 Sequestered: Design and Construction of Synthetic Organelles Co‐localization of a pathway also ensures substrate channeli ...

14.2 On Organelles 281 14.2 On Organelles Advances in imaging and comparative genomics have muddled the latter twenti­ eth‐centu ...

282 14 Sequestered: Design and Construction of Synthetic Organelles Catalysis is often the motivating factor for organelle engin ...

14.3 ­roteinnBased Organelles 283 extreme – that is, if you can’t build it, you don’t understand it. From a historical perspecti ...

284 14 Sequestered: Design and Construction of Synthetic Organelles Examples of these proteins are CcmK2 and CsoS1A from the CB, ...

14.3 ­roteinnBased Organelles 285 of the shell. Depending on the BMC, there are thousands of copies of protomers (i.e., ~2000 Ru ...

286 14 Sequestered: Design and Construction of Synthetic Organelles signal sequence can interact with the hexameric shell protei ...

14.3 ­roteinnBased Organelles 287 work is required to understand the true role of CsoS2 and to develop a strategy to target fore ...

288 14 Sequestered: Design and Construction of Synthetic Organelles have solved the structure of the CsoS1D protein from the α‐C ...

14.3 ­roteinnBased Organelles 289 extremely high [11]. Interestingly, there is little information on the effect such harsh condi ...

290 14 Sequestered: Design and Construction of Synthetic Organelles 10 genes from H. neapolitanus led to fully formed CBs with a ...

14.3 ­roteinnBased Organelles 291 Encapsulins (also called nanocompartments) are a family of poorly charac­ terized proteins tha ...

292 14 Sequestered: Design and Construction of Synthetic Organelles threefold axis pores – they are positively charged in the Th ...

14.4 ­ipidnBased Organelles 293 14.4.1 Repurposing Existing Organelles 14.4.1.1 The Mitochondrion The mitochondrion is the site ...