Synthetic Biology Parts, Devices and Applications

12.4 DNA rogram 253 changed, not only by changing the DNA program repeats on plasmid DNA but also by changing the number of plas ...

254 12 Metabolic Channeling Using DNA as a Scaffold in a consecutive manner. The arrangement [1 : 1 : 2] with an 8‐bp spacer pro ...

12.5 Applications of DNA‐Guided rogramming 255 Many biosynthetic pathways are also branched, which means that the enzymes at the ...

256 12 Metabolic Channeling Using DNA as a Scaffold DNA‐binding domains consequently defines the order of the enzymes of the met ...

References 257 4 Steen, E.J., Kang, Y., Bokinsky, G., Hu, Z., Schirmer, A., McClure, A., Del Cardayre, S.B., and Keasling, J.D. ...

258 12 Metabolic Channeling Using DNA as a Scaffold 15 Wilner, O.I., Weizmann, Y., Gill, R., Lioubashevski, O., Freeman, R., and ...

References 259 cell‐based selection. Proc. Natl. Acad. Sci. U.S.A., 100 , 12271–12276. http:// http://www.pubmedcentral.nih.gov/ ...

261 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...

262 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells tural and catalytic roles of RNA are due in large part to the tr ...

13.3 Design Principles for RNA Are Well Understood 263 [18]. Many metabolic genes are “switched” on or off, triggered by the bin ...

264 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 13.3.1 RNA Secondary Structure is Predictable Most RNAs fold int ...

13.3 Design Principles for RNA Are Well Understood 265 integrate user‐supplied experimental data such as selective 2′ hydroxylat ...

266 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells RNAs – ribozymes and riboswitches, respectively – underscore the ...

13.4 Applications of Designed RNA Scaffolds 267 remain poorly understood. Investigation of the dynamics of mRNA as it goes throu ...

268 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells strands comprising polymerization domains and aptamers for MS2 a ...

13.4 Applications of Designed RNA Scaffolds 269 which are effectively addressed by scaffolding [1]. However, the role of enzyme ...

270 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 13.5 Conclusion RNA is a powerful tool to synthetic biologists. ...

References 271 RNA imaging [119] can be further advanced by incorporation of docking sites that allow RNA to be probed with olig ...

272 13 Synthetic RNA Scaffolds for Spatial Engineering in Cells 8 Lorenz, R., Bernhart, S.H., Siederdissen, C.H.Z., Tafer, H., F ...

References 273 transcriptome‐wide RNA structure probing using high‐throughput sequencing. Nat. Methods, 7 (12), 995–1001. 28 Ker ...