Synthetic Biology Parts, Devices and Applications
170 8 Design of Ligand‐Controlled Genetic Switches Based on RNA Interference (triggers) and control RNAi‐mediated gene expressio ...
8.2 Development of RNAi Switches that Respond to rigger Molecules 171 Hereafter, we will focus on an RNA design strategy and th ...
Table 8.1 Developed RNAi switches. References An et al . [10] Beisel et al . [11] Kumar et al. [12] Masu et al . [13] Beisel et ...
8.2 Development of RNAi Switches that Respond to rigger Molecules 173 switches), and the theophylline aptamer was introduced in ...
174 8 Design of Ligand‐Controlled Genetic Switches Based on RNA Interference inhibitory sequence, resulting in perfect hybridiza ...
8.4 Application of the RNAi Switches 175 pair lengths and introducing mutations [11, 15, 22–25]. Several ligand‐ (e.g., small mo ...
176 8 Design of Ligand‐Controlled Genetic Switches Based on RNA Interference RNP motif Tr igger protein Top view RNA motif (a) ( ...
8.5 Future Perspectives 177 bloodstream, efficient uptake by tumors, and reassociation‐triggered activa- tion of split siRNA fun ...
178 8 Design of Ligand‐Controlled Genetic Switches Based on RNA Interference Definitions RNAi RNA interference. The term is used ...
References 179 14 Beisel, C.L., Chen, Y.Y., Culler, S.J., Hoff, K.G. et al. (2011) Design of small molecule‐responsive microRNAs ...
181 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...
182 9 Small Molecule-Responsive RNA Switches (Bacteria) aptamers against numerous small molecules [6], isolating new aptamers fo ...
9.3 Mechanisms 183 of individual mutants. The Gallivan and the Hartig groups, among others, have taken this approach by evaluati ...
184 9 Small Molecule-Responsive RNA Switches (Bacteria) 9.3.2 Transcriptional Regulation Relative to translationally regulated r ...
Keywords with Definitions 185 9.4 Complex Riboswitches Although the majority of natural riboswitches provides a simple yet effic ...
186 9 Small Molecule-Responsive RNA Switches (Bacteria) Ribozyme An RNA sequence capable of catalyzing a chemical reaction such ...
References 187 16 Fowler, C.C., Brown, E.D., and Li, Y. (2008) A FACS‐based approach to engineering artificial riboswitches. Che ...
188 9 Small Molecule-Responsive RNA Switches (Bacteria) 33 Sharma, V., Nomura, Y., and Yokobayashi, Y. (2008) Engineering comple ...
189 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...
190 10 Programming Gene Expression by Engineering Transcript Stability Control and Processing in Bacteria could, therefore, incr ...
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