Synthetic Biology Parts, Devices and Applications
49 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH & ...
50 4 Rational Efforts to Streamline the Escherichia coli Genome living cells possess the intrinsic ability for physiological and ...
4.3 The E. coli Genome 51 genetically stable. Even if mutagenesis and evolvability cannot be totally repressed, genetic change s ...
52 4 Rational Efforts to Streamline the Escherichia coli Genome provides regulatory and other functions (~11%). The genome is th ...
4.3 The E. coli Genome 53 the genomic islands, they are called “core genome” and “auxiliary genome,” respectively. How many gen ...
54 4 Rational Efforts to Streamline the Escherichia coli Genome irrelevant under defined laboratory or industrial conditions. It ...
4.5 Selecting Deletion Targets 55 Rational, serial construction of targeted genomic deletions requires the full knowledge of the ...
56 4 Rational Efforts to Streamline the Escherichia coli Genome (“genes that have not been shown to be nonessential”; http://eco ...
4.5 Selecting Deletion Targets 57 In conclusion, genome streamlining is in large part a trial-and-error process. The large numbe ...
58 4 Rational Efforts to Streamline the Escherichia coli Genome they can be a nuisance in synthetic biology constructions. Delet ...
4.6 Targeted Deletion Techniiues 59 4.5.2.7 Genetic Diversity-Generating Factors SOS-induced translesion DNA polymerases (polII, ...
60 4 Rational Efforts to Streamline the Escherichia coli Genome In a second recombination event, the exogenous sequences can be ...
4.6 Targeted Deletion Techniiues 61 Selection of cointegrates Sequence to be deleted Cloning Electroporation AbR Plasmid integra ...
62 4 Rational Efforts to Streamline the Escherichia coli Genome 4.6.2.2 Linear DNA-Based Method A more straightforward method ap ...
4.6 Targeted Deletion Techniiues 63 I-SceI- stimulated scarless resolution. In principle, addition of new deletions to the final ...
64 4 Rational Efforts to Streamline the Escherichia coli Genome Recently, rapid streamlining and genome-wide inactivation of IS ...
4.7 GenomeeReducing Efforts and the Immact of Streamlining 65 recombinogenic or mobile DNA stabilized the MDS genomes and provid ...
66 4 Rational Efforts to Streamline the Escherichia coli Genome of a heterologous toxic protein. Versions of MDS42, rendering it ...
4.7 GenomeeReducing Efforts and the Immact of Streamlining 67 4.7.3 Complex Streamlining Efforts Based on Growth Properties In a ...
68 4 Rational Efforts to Streamline the Escherichia coli Genome 4.7.4 Additional Genome Reduction Studies In an early proof-of-c ...
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