Synthetic Biology Parts, Devices and Applications
110 6 Constitutive and Regulated Promoters in Yeast In this chapter, we deal with natural and synthetic promoters frequently use ...
6.2 Yeast Promoters 111 provides transcription initiation start sites (TSSs) defined by the consensus sequence A(Arich) 5 NPyA(A ...
112 6 Constitutive and Regulated Promoters in Yeast to several physiological stimuli, converging transcription initiation on the ...
6.3 Natural Yeast Promoters 113 position of these sequences along promoters have a direct effect on nucleosome occupancy and by ...
114 6 Constitutive and Regulated Promoters in Yeast the two open reading frames revealed the presence of a galactose‐dependent U ...
6.3 Natural Yeast Promoters 115 Carbon catabolite repression is the set of regulatory mechanisms forcing cells to preferentially ...
116 6 Constitutive and Regulated Promoters in Yeast The cytochrome c isoform 1 (CYC1) promoter has been extensively charac- teri ...
6.4 Synthetic Yeast Promoters 117 An alternative strategy for obtaining promoter libraries is the substitution of the non‐consen ...
118 6 Constitutive and Regulated Promoters in Yeast copies; in most cases, a linear relationship is observed [90, 99, 102–104]. ...
6.4 Synthetic Yeast Promoters 119 Hybrid promoters containing heterologous TFBSs need a heterologous tran- scription factor. To ...
120 6 Constitutive and Regulated Promoters in Yeast domain of Gal4 are separated and fused to the chromoprotein phytochrome PhyB ...
6.5 Conclusions 121 been applied to mammalian synthetic gene circuits [132]. Repression by steric hindrance can also be obtained ...
122 6 Constitutive and Regulated Promoters in Yeast Definitions Transcription initiation: Initial steps of transcription until t ...
References 123 10 Struhl, K. (1995) Yeast transcriptional regulatory mechanisms. Annu. Rev. Genet., 29 (1), 651–674. 11 Weake, V ...
124 6 Constitutive and Regulated Promoters in Yeast 28 Guarente, L. and Hoar, E. (1984) Upstream activation sites of the CYC1 ge ...
References 125 nucleosome disfavoring sequences allows fine‐tune regulation of gene expression in yeast. Nat. Genet., 44 (7), 74 ...
126 6 Constitutive and Regulated Promoters in Yeast 62 Fürst, P., Hu, S., Hackett, R., and Hamer, D. (1988) Copper activates met ...
References 127 79 Denis, C.L., Ferguson, J., and Young, E.T. (1983) mRNA levels for the fermentative alcohol dehydrogenase of Sa ...
128 6 Constitutive and Regulated Promoters in Yeast 94 Blake, W.J., Balázsi, G., Kohanski, M.A., Isaacs, F.J., Murphy, K.F., Kua ...
References 129 110 Dingermann, T., Frank‐Stoll, U., Werner, H., Wissmann, A., Hillen, W., Jacquet, M., and Marschalek, R. (1992) ...
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