Horticultural Reviews, Volume 44
ORCHID BIOTECHNOLOGY 197 of alginate are strongly viscous, even at 1% solute concentration (Chap- man and Chapman 1980). By ad ...
198 W. A. VENDRAME AND A. A. KHODDAMZADEH than 1 year without subculturing (Divakaran et al. 2006). PLBs ofCoel- ogyne breviscap ...
ORCHID BIOTECHNOLOGY 199 According to Villalobos and Engelmann (1995), over 1200 institu- tions worldwide have established ger ...
200 W. A. VENDRAME AND A. A. KHODDAMZADEH Classical techniques are generally operationally complex since they require the use of ...
ORCHID BIOTECHNOLOGY 201 Reed 2008). These includeCentaurium rigualii(Gonzalez-Benito and Perez 1997), alfalfa (Shibli et al. ...
Table 4.2. List of most relevant cryopreservation studies with orchids. Orchid species, cultivars,or hybrids Explant type Cryopr ...
Seidenfadenia mitrata Protocorm Vitrification 80 min PVS2 (25 ◦C ± ◦ 2 C) 67% Thammasiri (2008) Dendrobium candidum Protocorm En ...
Table 4.2. (Continued ) Orchid species, cultivars,or hybrids Explant type Cryopreservationtechnique z Survival (%) ofexplants po ...
Dactylorhiza fuchsii Seed Temperature 62 ◦C 0% Pritchard (1985) Guarianthe aurantiaca (syn. Cattleya aurantiaca ) Seed ◦ 5 C (wa ...
Table 4.2. (Continued ) Orchid species, cultivars,or hybrids Explant type Cryopreservationtechnique z Survival (%) ofexplants po ...
Dendrobium hercoglossum Seed Encapsulation-vitrification 80 min PVS2 (25 ◦C ± ◦ 2 C) 80% Thammasiri (2008) Phalaenopsis pulcherr ...
208 W. A. VENDRAME AND A. A. KHODDAMZADEH cryopreservation studies with 10 different orchid species showed that seeds recovered ...
ORCHID BIOTECHNOLOGY 209 Bratoniahybrid seeds. Best results were obtained in MS and Morel media (Morel 1960). The use of cryop ...
210 W. A. VENDRAME AND A. A. KHODDAMZADEH Early reports on pollen storage indicated that fresh pollen from differ- ent orchid sp ...
ORCHID BIOTECHNOLOGY 211 growers to improve production systems through clonal propagation as well as effectivein vitrogerminat ...
212 W. A. VENDRAME AND A. A. KHODDAMZADEH biology, Vol. 589. Protocols for in vitro propagation of ornamental plants. Humana Pre ...
ORCHID BIOTECHNOLOGY 213 Chang, C., and W.C. Chang. 2000. Micropropagation ofCymbidium ensifoliumvar. misericors through callu ...
214 W. A. VENDRAME AND A. A. KHODDAMZADEH Danso, K.E., and B.V. Ford-Lloyd. 2003. Encapsulation of nodal cuttings and shoot tips ...
ORCHID BIOTECHNOLOGY 215 Escobar, A. 1998. Whose knowledge, whose nature? Biodiversity, conservation, and the political ecolog ...
216 W. A. VENDRAME AND A. A. KHODDAMZADEH Gonzalez-Arnao, M.T., M.E. Martinez-Montero, C.A. Cruz-Cruz, and F. Engelmann. Advanc ...
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