Computational Systems Biology Methods and Protocols.7z

spectral assignment and integration. In addition, the existence of macromolecule in analytical samples could produce broader res ...

5.1 Raw Data Preprocessing and Metabolite Identification of MS Platform For GC-MS-based metabolomics, the main procedure of data ...

concentration, temperature, and excessive dilution. In general, spectra are initially aligned to an internal standard to remove ...

pathway metabolite involved are retrieved as a result. The features of different databases are summarized by Ellinger et al. [10 ...

technical errors) in metabolomics study. Currently, numerous methods are applied in the normalization of metabolomics [113–115], ...

Aside from the non-supervised approach, the supervised multivari- ate technique is utilized to identify interested metabolites d ...

study. They provide integrative metabolite information retrieving from public databases, such as KEGG, SMPDB, HMDB, Pub- Chem, a ...

generation of metabolite profile and the interpretation of mecha- nism underlying. In addition, combining with other omics tech- ...

Vuckovic D (2012) Current trends and chal- lenges in sample preparation for global meta- bolomics using liquid chromatography-m ...

Masson P, Alves AC, Ebbels TM, Nicholson JK, Want EJ (2010) Optimization and evalu- ation of metabolite extraction protocols fo ...

mass spectrometry for targeted metabolomics of neurochemicals in biological samples. J Chromatogr A 1446:78–90. https://doi. org ...

spectrometry with HILIC and RPLC separa- tions. Anal Chim Acta 650(1):3–9.https:// doi.org/10.1016/j.aca.2009.03.039 Dunn WB, E ...

Gheynst J, Fiehn O, Arita M (2015) MS-DIAL: data-independent MS/MS decon- volution for comprehensive metabolome analysis. Nat Me ...

customized carbon total correlation spectros- copy NMR metabolomics database. Anal Chem 84(21):9395–9401.https://doi.org/ 10.102 ...

Xia JG, Wishart DS (2010) MSEA: a web-based tool to identify biologically mean- ingful patterns in quantitative metabolomic dat ...

Chapter 17 Single-Cell Protein Assays: A Review Beiyuan Fan, Junbo Wang, Ying Xu, and Jian Chen Abstract Quantification of singl ...

cytometry, (4) microengraving, and (5) single-cell barcoding microchip. In this chapter, we examine the advantages and limita- t ...

Fig. 1Techniques enabling characterization of the protein expressions of single cells, which mainly include (a) flow cytometry, ...

Table 1 Quantitative copy numbers of single-cell proteins Technique Key results Flow cytometry Thy-1, Lyt-1, Lyt-2, and Lyt-3 of ...

Table 1 (continued) Technique Key results Flow cytometry CD19 molecules were quantified as 22 (normal B cells), 13 (chronic lymp ...