Computational Systems Biology Methods and Protocols.7z
Some studies provided solutions for increasing probe capture ability or reducing sequence distance between probes and target DNA ...
designed a new set of universal primers for vertebrate mitogenomes by referring its most conservative regions and then amplify m ...
Illumina platform in accordance with its library construction pro- tocol. The detailed LR-LCH protocol is as follows. This proto ...
3 Methods 3.1 Prepare Probe 1. Primes for amplifying mitogenome To achieve universality, we designed degenerate primers on the c ...
TF1 to TR1 amplicon is 5:8. Probe making is conducted in 50 μL reactions and mix the following reagents: (a) PCR product mixture ...
Library amplification is carried out in 100μL reactions and mix the following reagents: (a) Size-selected library. (b) 10μL5PC ...
Separate hybridized target molecules from the bait in 30μLTE by incubation at 95C for 5 min in a thermal cycler. The PCR condi ...
Table 2Results from Ion Torrent PGM of seven samples mixed togetherLibraryname a Total numberof reads Unique mappedreads (%) Err ...
the end ofND2, at the beginning ofND4andND5, at the end of ND5, and in control region. The capture sensitivity are 11.49% and 15 ...
Fig. 2 Coverage distributions for 480 bp and 2 kb library. ( a) represents Rana sp.2 results by using standard capture hybridiza ...
samples, DNA fragment with a length of 2 kb to cross-species capture is suitable for enrichment of high variable loci since the ...
entire koala retrovirus genome. PLoS One 9: e95633 Gasc C et al (2016) Sequence capture by hybri- dization to explore modern an ...
Chevalier FD et al (2014) Efficient linkage mapping using exome capture and extreme QTL in schistosome parasites. BMC Genomics ...
Chapter 4 The Introduction and Clinical Application of Cell-Free Tumor DNA Jun Li, Renzhong Liu, Cuihong Huang, Shifu Chen, and ...
circulating tumor biomarkers. And in section 3 and 4, we will introduce the ctDNA detection methods and its clinical application ...
occupying only 0.01% of total cell-free DNA (cfDNA)[8]. For exam- ple, for localized GI tumors [colorectal cancer (CRC), gastric ...
2.4 Circulating mRNA Several circulating mRNA candidates have been studied for CRC screening and prognosis after surgical resect ...
fluorophore, and a quencher. In the absence of the mutation, during the Scorpion PCR reaction, the quencher close to the fluorop ...
activating EGFR mutations in tumor tissue, revealed the status of EGFR in the plasma of 32 cases (72.7%; 95% CI, 58.0–83.6%) [27 ...
curable early-stage malignancies, there are still several barriers for cancer screening [33, 34]. Many tests are uncomfortable f ...
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