Science - USA (2022-06-10)
channel, linkers bridge the layers of the in- ner ring to coalesce scaffold nups into eight relatively rigid spokes that are fle ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 3of18 Fig. 2. Structural and biochemical analyses of the Nup192-Nic96 ...
and fig. S2, A and D). The Nic96R2sequence register was unambiguously assigned by iden- tifying seleno-L-methionine (SeMet)–labe ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 5of18 Fig. 3. Structural and biochemical analyses of the Nup192-Nup14 ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 6of18 Fig. 4. Structural and biochemical analyses of the Nup188-Nup14 ...
(F715A), moderately disrupted Nup188NTD binding (Fig. 4D and fig. S27). Further sys- tematic mutagenesis led to a Nup145N EDSILF ...
results, see the supplementary text (figs. S32 to S37). Together, these data elucidated the archi- tecture of theS. cerevisiaein ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 9of18 Fig. 5. Functional in vivo dissection of theS. cerevisiaeNPC li ...
amphipathicahelix, human NUP53R2binds the conserved NUP93SOLhydrophobic groove that encompassesahelicesa5anda13 toa 15 as a line ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 11 of 18 Fig. 6. Evolutionary conservation of the human linker-scaffo ...
figs. S79 and S80, D and E). NUP53 could also mediatelong-rangelinksbetweenthebridge NUP155 and the R1 and R2 binding sites on o ...
the accompanying manuscript) ( 60 ) sites are within reach of the ~115-residue linker be- tween the APD and the R1-R2-R3 regions ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 14 of 18 Fig. 8. Linker-scaffold architecture of the human NPC inner ...
out of reach. We used comprehensive residue- level biochemical reconstitution and mapping, crystallographic and single-particle ...
accommodate the passage of small cytosolic domains of inner nuclear membrane inte- gral membrane proteins (INM-IMPs), sug- gesti ...
J. Fischer, R. Teimer, S. Amlacher, R. Kunze, E. Hurt, Linker Nups connect the nuclear pore complex inner ring with the outer r ...
(NIH). GM/CA has been funded in whole or in part with federal funds from the National Cancer Institute (ACB-12002) and the Natio ...
RESEARCH ARTICLE ◥ QUANTUM COMPUTING Quantum advantage in learning from experiments Hsin-Yuan Huang1,2, Michael Broughton^3 , Jo ...
encodes how the classical memory changes as we obtain more experimental data. We then analyzed how the transitions on the tree d ...
based on conventional experiments, this ad- vantage was not proven against all possible algorithms in the conventional scenario. ...
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