Science - USA (2022-06-10)
previous reports, the RanBDs of NUP358 and NUP50 only bound Ran(GTP), whereas the ZnFs in NUP358 and NUP153 bound Ran in both nu ...
OE regions, whereas all other domains were dispensable (Fig. 5B and fig. S62). When NUP358NTDor NUP358OEwere tested in iso- lati ...
incubation periods andachieved only a partial NUP358 knockdown, potentially allowing sec- ondary phenotypes to emerge from prolo ...
to 100% of cells displayed a strong nuclear 5-EU–labeled RNA signal that decreased over time with a concomitant increase in the ...
NUP160::NG-AIDDLD1 cells after depletion of NUP160, the knockout of which causes RNA retention inS. cerevisiae,demonstrating the ...
cargo (Fig. 6, B and C, and fig. S83A). However, the NUP214NTD•DDX19 complex forms tighter interactions when DDX19 is in its ADP ...
Placement of the CFNC hub into the tube- like density puts CCS1, CCS2, and likely the unresolved CCS3 within reach of the ~40-Å ...
architectural outcome: TheC. thermophilum NPC presents two distinct assembly sensor motifs for the CFNC hub in the Nup37 and Nup ...
face cryo-ET density, accounting for ~23 MDa of structured mass. The more flexibly attached regions of the CF nups that are not ...
outstanding challenge for structural determi- nation. Nevertheless, our analysis has already identified that competition for bin ...
A. E. Hodelet al., The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleopor ...
635 – 652 (2000). doi:10.1083/jcb.148.4.635; pmid: 10684247 J. M. Cronshaw, A. N. Krutchinsky, W. Zhang, B. T. Chait, M. J. Mat ...
RESEARCH ARTICLE SUMMARY ◥ NUCLEAR PORE COMPLEX Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and ...
RESEARCH ARTICLE ◥ NUCLEAR PORE COMPLEX Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFo ...
nuclease to remove contaminating chromatin (fig. S1A). Cryo-EM data collection was con- ducted at different stage tilts and in c ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 3of11 Fig. 2. Fitting of Y-complex Nups with AlphaFold.(A) Cryo-EM den ...
the Nup358 N-terminal region as having a large⍺-helical domain (~800 residues), a linker, and an isolated single⍺-helix (Fig. 4, ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 5of11 Fig. 3. Interactions mediated by Nup205 and the Nup214-Nup88-Nup ...
of the CR assembly that was unexpected is the observed asymmetry in the composition and mode of binding among Nups: the con- for ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 7of11 Fig. 4. Nup358 interacts with the Y-complexes as clamps.(A) Doma ...
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