Nature - USA (2020-02-13)
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290 | Nature | Vol 578 | 13 February 2020 Article The guidance receptor plexin D1 is a mechanosensor in endothelial cells Vedant ...
Nature | Vol 578 | 13 February 2020 | 291 transcription factors that are known to be upregulated by atheropro- tective shear str ...
292 | Nature | Vol 578 | 13 February 2020 Article was a global cell-wide phenomenon. Fourth, we examined the phos- phorylation o ...
Nature | Vol 578 | 13 February 2020 | 293 Cell–cell and cell–matrix adhesions represent two sites that are highly mechanically a ...
294 | Nature | Vol 578 | 13 February 2020 Article provide evidence that PLXND1 is necessary and sufficient for the shear-stress- ...
Nature | Vol 578 | 13 February 2020 | 295 Liu, Y., Sweet, D. T., Irani-Tehrani, M., Maeda, N. & Tzima, E. Shc coordinates s ...
Article Methods Data reporting No statistical methods were used to predetermine sample size. Mouse experiments were randomized. ...
and descending aorta segments were incubated with primary anti- bodies (PLXND1 (PA5-21605, Thermo Fisher Scientific) and PECAM-1 ...
Article Fluor 488 C5 maleimide (Thermo Fisher Scientific), and the reaction was allowed to proceed for 24 h at 6 °C in the dark. ...
Extended Data Fig. 1 | Knockout or knockdown of PLXND1 and other genes in ECs. a–f, ECs were either isolated from Plxnd1fl/fl an ...
Article Extended Data Fig. 2 | PLXND1 mediates the EC response to f luid shear stress. a, BAECs were transfected with scrambled ...
Extended Data Fig. 3 | Mechanotransduction by PLXND1 is independent of its ligand-binding functions. a, BAECs were treated with ...
Article Extended Data Fig. 4 | Lipid-prof ile analysis and expression of inf lammatory markers in the aortic arch. a, Body weigh ...
Extended Data Fig. 5 | Atherosclerosis in the descending aorta. a, Representative en face preparations of the whole aorta showin ...
Article Extended Data Fig. 6 | See next page for caption. ...
Extended Data Fig. 6 | Mechanical force on PLXND1 results in integrin activation, whereas ligand stimulation causes ECs to colla ...
Article Extended Data Fig. 7 | See next page for caption. ...
Extended Data Fig. 7 | PLXND1 colocalizes and associates with members of the junctional mechanosensory complex, and its levels a ...
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