Nature - USA (2020-02-13)
Article Extended Data Fig. 8 | See next page for caption. ...
Extended Data Fig. 8 | Relationship between PLXND1 and other established mechanosensors. a, Pecam1+/+ and Pecam1−/− ECs were inc ...
Article Extended Data Fig. 9 | Force application on other members of the PLXND1 mechanocomplex does not elicit a mechanotransduc ...
Extended Data Fig. 10 | Validation of the PLXND1 mutant. a, Negative-stain two-dimensional class averages of wild-type PLXND1 we ...
Article Extended Data Fig. 11 | An open conformation of PLXND1 is required for force-dependent signalling. Mouse lung ECs in whi ...
1 nature research | reporting summary April 2018 Corresponding author(s): Ellie Tzima Reporting Summary Nature Research wishes t ...
2 nature research | reporting summary April 2018 Data Policy information about availability of data All manuscripts must include ...
3 nature research | reporting summary April 2018 1:1000), pAktS473 (4060, Cell Signaling Technology, 1:1000), tAkt (4691, Cell S ...
296 | Nature | Vol 578 | 13 February 2020 Article Stress- and ubiquitylation-dependent phase separation of the proteasome Sayaka ...
Nature | Vol 578 | 13 February 2020 | 297 the course of 3 h. When the cells were treated with the proteasome inhibitor MG-132 or ...
298 | Nature | Vol 578 | 13 February 2020 Article in a proteasome activity-dependent manner after 4 h (Extended Data Fig. 4c, d) ...
Nature | Vol 578 | 13 February 2020 | 299 proteasomes were rapidly exchanged into and out of the foci, further supporting the id ...
300 | Nature | Vol 578 | 13 February 2020 Article RAD23B drives LLPS of ubiquitin chains These results raised the possibility th ...
Methods No statistical methods were used to predetermine sample size. The experiments were not randomized. The investigators wer ...
Article Virus particles were produced in HEK293T cells co-transfected with Gag-Pol, VSV-G, and RAD23B retrovirus plasmids in six ...
(Tomy Seiko), and then collected by centrifugation (20,000g for 15 min at 4 °C). The protein concentration was determined using ...
Article Propidium iodide exclusion assay and measurement of caspase-3 activity Cell death under hyperosmotic stress was quantifi ...
DMEM supplemented with penicillin–streptomycin, 1% non-essential amino acids and 10% fetal bovine serum overnight. Thirty minute ...
Article Extended Data Fig. 1 | Characterization of KI cells stably expressing f luorescent protein-labelled proteasome subunit o ...
Extended Data Fig. 2 | Proteasome foci formation occurs in various cell types following hyperosmotic stress. a, Localization of ...
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