BIOINORGANIC CHEMISTRY A Short Course Second Edition

240 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II nucleic acid – base interactions but also on interactions between ribo ...

MAGNESIUM AND CATALYTIC RNA 241 position) for RNA as the substrate segment. These experiments teased out some mechanistic detail ...

242 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II Reference 16 discusses biochemical methods to predict specifi c ribozy ...

MAGNESIUM AND CATALYTIC RNA 243 and transition states. Therefore, one might have an active site in one conforma- tion that uses ...

244 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II fying pro - chiral atoms will be important in the discussion of the gr ...

MAGNESIUM AND CATALYTIC RNA 245 Tetrahymena pre - rRNA, beginning in 1982. Subsequent work showed that a specifi c guanosine bin ...

246 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II are defi ned as joining regions. The catalytic core is located at the ...

MAGNESIUM AND CATALYTIC RNA 247 The guanosine binding site of group I introns is located within P7 — a six - base - pair helix w ...

248 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II tetraloop and its receptor. The end result is that a number of riboses ...

MAGNESIUM AND CATALYTIC RNA 249 However, hydrogen - bonding characteristics could not be defi ned, nor could magnesium ions be p ...

250 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II present a model for the 5 ′ splice site based on previous functional g ...

MAGNESIUM AND CATALYTIC RNA 251 group I intron crystal structures exhibit much more agreement between bio- chemical and spectros ...

252 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II PDB: 1GID structure. (See Table 6.1 .) The PDB: 1HR2 bond length data ...

MAGNESIUM AND CATALYTIC RNA 253 Another base triple between the bulged - out A218 in P6 and the P3 domain base pair C102 – G272 ...

254 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II (Ir)] models were used to place magnesium ions in the fi nal native st ...

MAGNESIUM AND CATALYTIC RNA 255 the Scott Strobel research group (PDB: 1U6B).^22 This 222 nucleotide structure included theAzoar ...

256 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II In the PDB: 1U6B structure the 3 ′ - exon is held in place through int ...

MAGNESIUM AND CATALYTIC RNA 257 tide forms the only interaction holding the 5 ′ - exon in place because any cova- lent bond betw ...

258 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II A more detailed crystal structure for the Azoarcus sp. ribozyme was pu ...

MAGNESIUM AND CATALYTIC RNA 259 by the Cech group in 2004, one metal ion (possibly Mg 22 +) coordinated to the O2′ and O3 ′ of ω ...