BIOINORGANIC CHEMISTRY A Short Course Second Edition

260 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II metal ion mechanism proposed for PDB: 1ZZN is similar to that seen for ...

MAGNESIUM AND CATALYTIC RNA 261 conserved features in other RNA – RNA or RNA – protein structures. For instance, the Twort guano ...

262 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II consisting of single - stranded RNA. They are smaller and simpler than ...

MAGNESIUM AND CATALYTIC RNA 263 hammerhead ribozyme crystallographic studies. Domain 1 (or I) contains a U - turn motif (similar ...

264 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II step; and (3) the dissociation of the ribozyme products. The cleavage ...

MAGNESIUM AND CATALYTIC RNA 265 The structure showed that the three base - paired stems I, II, and III form type A - DNA helices ...

266 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II out at the phosphate 5 ′ to the cleavage site, but this site was at le ...

MAGNESIUM AND CATALYTIC RNA 267 TABLE 6.3 Description of Hammerhead Crystal Structures PDB ID Description Metal Ions (Locations) ...

268 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II coordination of a metal cation to the A 9 – G 10.1 Me 2+ binding site ...

MAGNESIUM AND CATALYTIC RNA 269 attachment. If the added ligands arose from additional interactions with RNA nucleobases, ribose ...

270 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II TABLE 6.4 Selected Bond Distances for X Ray Crystallographic Structur ...

MAGNESIUM AND CATALYTIC RNA 271 Selected Bond Distances PDB: 301D ( Å ) pH = 8.5 A9 – G10.1 region Mg 2+ – O Mg 2+ N N G 7 10. ...

272 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II in position is accompanied by other nucleobase and ribose movements wi ...

MAGNESIUM AND CATALYTIC RNA 273 whether catalytic activity can be recovered (rescued). In 1996, the group pub- lished a paper in ...

274 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II of the base forming the mutant causes structural deformation in the ri ...

MAGNESIUM AND CATALYTIC RNA 275 In a 1998 RNA paper, the Herschlag group further examined base rescue at four positions: C 3 , A ...

276 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II Additional interactions and rearrangements in the transition state wit ...

MAGNESIUM AND CATALYTIC RNA 277 seems to be important because it is held in position by four hydrogen bonds to G 8 and A 9 and s ...

278 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II displaces a Mg 2+ ion that is essential for catalysis. The fi rst hypo ...

MAGNESIUM AND CATALYTIC RNA 279 First, the researchers found that the rate of substrate turnover in the talo - 5 ′ - C - methyl ...