BIOINORGANIC CHEMISTRY A Short Course Second Edition

280 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II Researchers in the fi eld continued to study the hammerhead ribozyme – ...

MAGNESIUM AND CATALYTIC RNA 281 (or both) site(s), A 9 /G 10.1 or G 1.1 /C 17 , had a deleterious effect on substrate cleav- age ...

282 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II P1.1 pro - R p phosphoryl oxygen – sulfur substitution consistent with ...

MAGNESIUM AND CATALYTIC RNA 283 site is critical for hammerhead catalytic activity and that another divalent metal site is proba ...

284 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II hammerhead – metal ion interactions that stimulate catalytic rate in t ...

MAGNESIUM AND CATALYTIC RNA 285 phate linkage to exist, although the one hydrogen bond seen in the crystal structure pairing U 1 ...

286 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II (A 6 N 6 – O 1P = 2.7 Å ) and (2) the close approach of G 5 ’ s keto o ...

MAGNESIUM AND CATALYTIC RNA 287 atom is positioned approximately 3 Å from the attacked phosphorus atom. This situation is assign ...

288 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II hammerhead RNAs at the same pH 6. The reference 55 researchers also fo ...

MAGNESIUM AND CATALYTIC RNA 289 metal ion binding to both the A 9 /G 10.1 and the cleavage site resulted in an unlikely high - e ...

290 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II by RNA folding they may not appear on the assay or will appear at lowe ...

MAGNESIUM AND CATALYTIC RNA 291 tration over a range of Mg 2+ or Li + concentrations from 0.1 to 100 mM. The importance of the 2 ...

292 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II and C 1.2 in the substrate, are highly solvent accessible in the X - r ...

MAGNESIUM AND CATALYTIC RNA 293 5 ′ – 5 ′ - connected base on stem I formed a Watson – Crick base pair with C 10.4 creating a te ...

294 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II during crystallization creates a kinetic bottleneck preventing cleavag ...

MAGNESIUM AND CATALYTIC RNA 295 TABLE 6.6 Inline Fitness Data for Hammerhead Ribozyme Crystal Structures a PDB Accession Number ...

296 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II could constitute a biochemical switch that might control whether the R ...

MAGNESIUM AND CATALYTIC RNA 297 information on function and mechanism of catalysis. In contrast to the success of this method fo ...

298 GROUP I AND II METALS IN BIOLOGICAL SYSTEMS: GROUP II ion to the essential N 2 , N 1 , or O 6 positions of the G 5.0 guanosi ...

MAGNESIUM AND CATALYTIC RNA 299 and A 14. As mentioned many times before, X 1.1 and A 9 are important metal ion binding sites. W ...