Cell - 8 September 2016
CD hsp-16.2p::GFP (with hsp-6 RNAi) Control Brain CM C16 C20 C22 C24 hsp-16.2p::GFP Control Heart CL C14 C18 A Control(EV) EV: h ...
We tested whether exogenous cardiolipin was sufficient to drive expression of the MCSR. We found that ectopic feeding of cardiol ...
reduced polyglutamine protein aggregation in the primary human fibroblast cell lines (Figure 7C). In addition, triglyceride leve ...
feeding was dependent on bothdve-1andhsf-1(Figure S5F), it is possible that cardiolipin accumulation serves as an initial signal ...
protein-folding landscape of the cytoplasm, gross changes in cellular lipid composition, or the presence of as little as a singl ...
BPerhexiline Treatment BMotility Assay BFeeding Lipids to Worms BHeat Shock Treatment dQUANTIFICATION AND STATISTICAL ANALYSIS B ...
Kim, D., Ouyang, H., and Li, G.C. (1995). Heat shock protein hsp70 accelerates the recovery of heat-shocked mammalian cells thro ...
STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NDUFS3 Abcam Ab14711; RRID: AB_301429 mtHSP70 ...
CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagents may be directed to, and will be fulfilled ...
were grown at 15C until they were gravid adults. Then, the cohorts were shifted to 25C. Day 1 adult worms were transferred to ...
Electron Microscopy 300-500 worms grown onE. coliHT115 carrying RNAi, were loaded into specimen carriers and fixed using high pr ...
Preparation of Palmitate–BSA Conjugate Palmitate was conjugated to BSA as described (Seahorse protocols). Briefly, sodium palmit ...
Sample Triglyceride control (C)=B/VXDnmol/mlormM Where:Bis the amount of triglyceride from standard curve (nmol) Vis the sample ...
Supplemental Figures C 0 50 100 150 200 250 300 EV hsp-6 dnj-10 dnj-21 spg-7 phb-2 grpE hsp-90 timm-8 timm-13 timm-17 timm-44 to ...
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 stdh-2 fat-5 acl-12 cpt-3 Heat Shock cco-1 RNAi mRNA Fold induction (Normalized to untreated Emp ...
A Electron Microscopy Control (EV) hsp-6 RNAi Control (EV) EV:hsp-6 hsp-6:dve-1 hsp-6:hsf-1 Double RNAi with hsp-6 B EV hsp-6 hs ...
Figure S3. Electron Microscopy and Nile Red Staining, Related toFigure 3 (A) More electron microscopy pictures ofC. elegansintes ...
0 200 400 600 800 1000 1200 1400 Control (EV) pod-2 fasn-1 haf-1 GFP Fluorescence (RFU) Control Heat Shock A D hsp-6p::GFP hsp-6 ...
(D) PHX induces mitochondrialhsp-6andhsp-60expression.hsp-6p::GFP and reporterhsp-60p::GFP induction was measured by COPAS bioso ...
A Control(EV) EV: hsp-6 hsp-6: crls-1RNAi Nonyl Acridine Orange Staining B Nile Red Staining Control Heart CL C14 C18 hsp-6p::GF ...
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