AMPK Methods and Protocols

Fig. 2Pax7Ki67MyoD labeling and analysis. MuSCs were extracted from total hind limbs as described. (a) Pax7Ki67MyoD labeling was ...

cells), labeling for Pax7 (muscle stem marker), Ki67 (proliferation marker) and MyoD (activation marker and commitment into the ...

Matrigel atþ 4 C for 4 h. Just before use, dilute it at 1/10 in cold DMEM/F-12 and keep on ice. To dissolve Ultroser™G (Pall C ...

References Mounier R, Chretien F, Chazaud B (2011) Blood vessels and the satellite cell niche. Curr Top Dev Biol 96:121–138 Kua ...

Chapter 35 Evaluating the Role of Host AMPK inLeishmaniaBurden Diana Moreira, Je ́roˆme Estaquier, Anabela Cordeiro-da-Silva, an ...

infection (Subheading3.1). This differentiation procedure leads to a higher yield and reproducibility, being these bone marrow- ...

M-CrenegSirt1flox/flox) mice. All animals used in experiments are aged from 6 to 12 weeks. 2.L. infantum(MHOM/MA/67/ITMAP-263) p ...

Ethanol 75% (c/v) in H 2 O. H 2 O RNase free. RNeasy micro kit. QIAmp DNA micro kit. cDNA synthesis Kit. SYBR Green Supermix. P ...

2.7 Equipment 1. Gamma irradiator. Flow cytometer. Western blot transfer system. Microplate reader. Western blot detection syst ...

At day 5 of growth, washL. infantumpromastigotes twice with 5 mL of PBS and resuspend the parasites in 1 mL of PBS. Count the p ...

3.2 Infection of Mice with L. infantum and Sorting of Infected Splenic Macrophages 3.2.1 In Vivo Infection Infect mice intraper ...

Label the remaining cell suspension with 2 μg/ml anti- CD11b-PE, anti-Ly6C-PerCP/Cy5.5 and anti-Ly6G-Pacific Blue diluted in 25 ...

After 24 h of infection, detach BMMos by using the DMEM- EDTA solution and centrifuge at 300
gfor 10 min at room temperature. R ...

Obtain the non-mitochondrial respiration by subtracting the rotenone/antimycin A values. Calculate the spare respiratory capaci ...

higher sensitivity (0.01 parasites) than the qPCR (0.6 parasites) to confirm the positivity of the quantitative result. For the ...

The procedure warrants a homogeneous distribution of the cells under the XF-24 plate surface, decreasing the variability among ...

Verbeuren TJ, Cohen RA, Zang M (2008) SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinas ...

Chapter 36 Analysis of Transgenerational Phenotypes Following Acute Starvation in AMPK-DeficientC.elegans Emilie Demoinet and Ri ...

impinge on gene expression of the affected individuals [5–8]. More- over, these changes were further correlated with an increase ...

metabolism is maintained at a low level, as is overall gene expres- sion. Cell divisions do not occur, nor do L1 specific cellul ...