AMPK Methods and Protocols

and 0.025 M phosphate (Na 2 PO 4 ·H 2 O, pH 7.2). Formaldehyde is toxic so prepare this and use it in a fume hood (seeNote 1). ...

3 Methods 3.1 Effects on the Parental (P 0 ) Generation 3.1.1 Alkaline Hypochlorite Treatment and L1 Survival Defrost strains a ...

postembryonic development. L1 larvae are very small and fragile and are often best manipulated in liquid. Every 48 h, deposit 1 ...

Record the number of plates that still contain single animals 96 h after being transferred as L4: these animals can be consider ...

3.2 Transgenerational Defects 3.2.1 Multigenerational Analysis of Brood Size Both sterility and brood size can be assessed at ev ...

progeny, in this first selection, we assessed brood size in descen- dants from AMPK mutant parents that had a reduced number of ...

visibly unaffected parents (those that generated more than 100 F 1 progeny), and then using the same type of multigenera- tional ...

Allow these animals to reproduce, and evaluate their brood size by counting the number of F 2 animals. Once again, randomly sel ...

Deposit 5–10μL of resuspended L1 larvae in water on to the coated slides (3–4 slides per condition, 2 spots containing L1 larva ...

piece of Parafilm as above, and leave them in a dark humid chamber for 2–3 h at room temperature. Wash the slides three times i ...

Therefore, it is suggested that the L1 larvae be diluted in sterile M9 buffer with a density of 6–10 L1 larvae/μL and constant ...

References Feil R, Fraga MF (2012) Epigenetics and the environment: emerging patterns and implica- tions. Nat Rev Genet 13:97.h ...

Chapter 37 Humanγ2-AMPK Mutations Arash Yavari, Dhruv Sarma, and Eduardo B. Sternick Abstract In humans, dominant mutations in t ...

contractile unit of myocytes—prompting the conceptualization of HCM as a “disease of the sarcomere” [4, 5]. Comprehensive geneti ...

variably styledPRKAG2cardiac syndrome,PRKAG2cardiomyop- athy, orPRKAG2disease—these seminal reports fuelled intense scientific i ...

Table 1 Reported humanPRKAG2variants linked to a cardiomyopathy phenotype Variant Onset Number of families/ patients Key feature ...

Table 1 (continued) Variant Onset Number of families/ patients Key features Reference p.His344Pro Adult Family of two LVH and he ...

purebred Hampshire pigs (denoted RN
)[ 19] and the first described naturally occurring humanγ3 mutation (Arg225Trp) [20], both o ...

gene product, absence of the variant from control population data- bases (e.g., Exome Sequencing Project, 1000 Genomes Project, ...

fibrillation and atrial flutter have been described from the age of 2 years (but with no LVH even up to age of 41 years) in asso ...