Esophageal Adenocarcinoma Methods and Protocols
196 Table 1 Amplification or deletion of genes involved in esophageal adenocarcinoma Amplification/ Deletion Gene Ref. Amplifica ...
197 Table 1 (continued) Amplification/ Deletion Gene Ref. Immediate early response 3 (IER3) [ 14 ] Insulin like growth factor 1 ...
198 Table 1 (continued) Amplification/ Deletion Gene Ref. TNF receptor superfamily member 6b (TNFRSF6B) [ 13 ] Topoisomerase (DN ...
199 more sensitive tumor response marker in comparison to the changes in morphological tumor phenotype. For example, patients wh ...
200 HER-2 has a higher survival rate when treated with anti-HER-2 targeted therapy (trastuzumab) [ 48 , 49 ]. Therefore, further ...
201 Snap frozen cancer tissue and adjacent nonneoplastic tissues from patients with esophageal adenocarcinoma. 100% undenatured ...
202 Droplet generator (e.g., QX200 Droplet Generator). Droplet reader (e.g., QX200 Droplet Reader). Microfluidic cartridge. Gas ...
203 Optimize the annealing temperature of the primer sets designed by running a conventional PCR with gradient temperature. PCR ...
204 CAUTION! Conical flask may be hot when removing from the microwave oven. Ensure that protective heat gloves are worn when ha ...
205 Amplify the sample to end point with a gradient enabled PCR machine using the following cycling conditions (see Note 14): 3 ...
206 Fig. 3 Multiple target detection (genes of interest) using EvaGreen-based ddPCR. (a) One-dimensional droplet plots of result ...
207 4 Notes Ensure that there is no folding of tissue when placing the sec- tion onto the slide. Also, ensure that the section ...
208 Ramp rate for the entire cycle is set to 2 °C/s to ensure even heat distribution throughout the droplets. REMEMBER! Set the ...
209 AC, Chiang DY, Chirieac LR, Cho J, Fujii Y, Gazdar AF, Giordano T, Greulich H, Hanna M, Johnson BE, Kris MG, Lash A, Lin L, ...
210 https://doi.org/10.1158/1055-9965. epi-07-2734 Miller CT, Aggarwal S, Lin TK, Dagenais SL, Contreras JI, Orringer MB, Glove ...
211 Zhou Z, Xia Y, Bandla S, Zakharov V, Wu S, Peters J, Godfrey TE, Sun J (2014) Vitamin D receptor is highly expressed in pre ...
212 Li X, Galipeau PC, Paulson TG, Sanchez CA, Arnaudo J, Liu K, Sather CL, Kostadinov RL, Odze RD, Kuhner MK, Maley CC, Self S ...
213 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
214 tissue. Similarly, increased methylation of cyclin-dependent kinase inhibitor 2A (CDKN2A) depends on the pathological stage ...
215 Gene Implications in esophageal adenocarcinoma Ref. ERBB2 Amplification, substitution, or truncation of receptor tyrosine-pr ...
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