Esophageal Adenocarcinoma Methods and Protocols
116 and unremarkable glands could be found. However, in TMAs, despite the small tissue size, they have the most representative t ...
117 Manual method is relatively easy to be performed and less time consuming than the semi-automated method. On the other hand, ...
118 Limitations of tissue microarrays in the evalu- ation of focal alterations of bcl-2 and p53 in whole mount derived prostate ...
119 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
120 tric/esophagogastric junction adenocarcinoma [ 11 ]. Trastuzumab has emerged as a novel treatment option for patients with H ...
121 The samples that may be tested are endoscopic esophageal biop- sies, esophagostomy specimens, and specimens from metastatic ...
122 Several reports have shown the effect of time to fixation (cold ischemia time), fixation time, or fixative type on the resul ...
123 3 Methods Several laboratory techniques are available to determine HER2 sta- tus. Gene amplification, which is the fundament ...
124 and in situ hybridization assays. The American Society of Clinical Oncology/College of American Pathologists (ASCO/ CAP) gui ...
125 The most commonly used method is immunohistochemistry, which is a low-cost technique that can be performed on small formalin ...
126 ●● CC2—Extended at 100 °C (HIER) ●● (ii) (ii) ISH—Protease 3 for 16 min (Enzyme Epitope Retrieval). ●● Denaturation—20 min. ...
127 a FISH slide. It is also an advantage for the pathologists who prefer the light microscopes. Staining protocol for Dako HER2 ...
128 may be influenced by pre-analytical issues as previously dis- cussed. Hence, it is imperative that validation and optimizati ...
129 A discrete signal is counted as a single copy of HER2 or Chr17. SISH signals (black) are typically smaller and more dis- cre ...
130 have a ratio more than 2 [ 47 ]. Recent studies have documented experience of false-positive IHC results due to interpretati ...
131 the IHC “1+” or “0” and FISH-positive group [ 11 ]. Others have found some negative cases by immunohistochemistry showed a l ...
132 Ben-Levy R, Paterson HF, Marshall CJ, Yarden Y (1994) A single autophosphorylation site confers oncogenicity to the Neu/Erb ...
133 Schoppmann SF, Jesch B, Zacherl J, Wrba F, Hejna M, Maresch J, Langer FB, Riegler MF, Pluschnig U, Birner P (2011) HER-2 st ...
134 T (2008) Assessment of a HER2 scoring sys- tem for gastric cancer: results from a validation study. Histopathology 52:797–80 ...
135 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
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