Esophageal Adenocarcinoma Methods and Protocols
136 Whole-slide imaging was used in assessment of DNA content of Feulgen-stained sections of Barrett esophagus with dysplasia [ ...
137 3 Methods ●● Turn on the power of scanner and then switch on computer monitor. ●● When the status screen indicates “scanner ...
138 Fig. 2 The program shows the slide rack position and slide order Fig. 3 After snapshot, the slide for scanning is shown on t ...
139 ●● Adjust the preselected areas in each slide to be scanned (see Note 4) (Fig. 4 ). ●● Make sure the areas to be scanned hav ...
140 ●● Press the Eject button, open the scanner door and collect the slides from the autoloader slide rack. ●● Log off the syste ...
141 4 Notes If the glass cover is too thick, use manual focusing and new focus points are selected which are relatively closer ...
142 cytometry: a novel method to detect abnormal DNA content in Barrett’s esophagus. Lab Investig 95:1319–1330. https://doi.org/ ...
143 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
144 esophagus. It is difficult to investigate the developmental progres- sion and natural history of Barrett’s esophagus–esophag ...
145 opment of esophageal adenocarcinoma (i.e., 50%), only 4/12 mice survived after 24 weeks of observation. Unfortunately, the a ...
146 In the upcoming sections, we detail three representative surgi- cal procedures: esophagojejunostomy (in mice with DgER), eso ...
147 Anastomose the distal esophagus to the jejunum at the enter- ostomy site. This will be an end-to-side anastomosis, with 4–8 ...
148 Fig. 3 Esophagogastroduodenal anastomosis with gastrectomy (used with per- mission from Norton Thoracic Institute, Phoenix, ...
149 SEER Cancer Statistics Review, 1975–2013. National Cancer Institute, Bethesda, MD; 2016 [Apr 2016]. Available from: http:// ...
150 development of esophageal adenocarcinoma in mice with jejunesophageal reflux. J Surg Res 83(1):48–55 Lechpammer M, Xu X, El ...
151 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
152 Established cancer cell lines play a central role in cancer cell biology investigations and in the development of novel anti ...
153 of the major obstacles in developing novel therapies for patients with esophageal adenocarcinoma has been the lack of an ani ...
154 3 Methods Before commencing cell culture, disinfect the hood cabinet with 70% ethanol. Disinfect gloves by spraying them wi ...
155 Quench trypsin by adding at least 3 volumes of 10% fetal bovine serum (FBS) containing medium (see Note 21). Pellet cells b ...
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